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Prøveforberedelse til hurtig lipidanalyse i Drosophila-hjernen ved hjælp af matrixassisteret laserdesorption / ioniseringsmassespektrometribilleddannelse

Article DOI: 10.3791/63930-v • 09:00 min • July 14th, 2022
July 14th, 2022 •
*1,5,6, *1,2, *3, 1,4, 7, 1,4,5, 1,4,5, 8, 9,10, 2, 1
1Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York, 2Advanced Science Research Center, Structural Biology Initiative, the City University of New York, Graduate Center, 3National Institute of Neurological Disorders and Stroke, National Institutes of Health, 4The City College of New York, CUNY, 5Macaulay Honors College, CUNY, 6Graduate School of Public Health and Health Policy, The City University of New York, 7Princeton University, 8Ardrey Kell High School, 9The Borough of Manhattan Community College, CUNY, 10Gallatin School of Individualized Study, New York University
* These authors contributed equally

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Formålet med denne protokol er at give detaljeret vejledning om korrekt prøveforberedelse til lipid- og metabolitanalyse i små væv, såsom Drosophila-hjernen , ved hjælp af matrixassisteret laserdesorption / ionisering (MALDI) massespektrometribilleddannelse.

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Biologi Udgave 185 MALDI MSI lipidprofilering Drosophila hjerne prøveforberedelse massespektrometri
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