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使用遗传密码扩增对细菌分泌蛋白进行超分辨率成像
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使用遗传密码扩增对细菌分泌蛋白进行超分辨率成像

Article DOI: 10.3791/64382-v • 13:11 min • February 10th, 2023
February 10th, 2023 •
1, 1
1Biochemistry & Molecular Biology, University of Texas Medical Branch

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本文提供了一种简单明了的方案，使用遗传密码扩展 （GCE） 位点特异性标记 沙门氏菌 分泌效应子，并使用直接随机光学重建显微镜 （dSTORM） 对 HeLa 细胞中分泌蛋白的亚细胞定位进行成像

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生物学，第 192 期， 沙门氏菌，非规范氨基酸，遗传密码扩展，SseJ，超分辨率，dSTORM
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