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JoVE Journal Biology
Microvasculaire en macrovasculaire endotheliale celisolatie en zuivering van longmonsters
Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting (Video) | JoVE
Journal (Biology)
Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting (Video) | JoVE
Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Journal (Biology)
Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
 
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Microvasculaire en macrovasculaire endotheliale celisolatie en zuivering van longmonsters

Article DOI: 10.3791/64885-v • 05:26 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 2, 2,3, 2,3, 1, 4, 4,5, 1,2, 2,3, 1, 6,7, 1, 1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS

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Hoewel uitdagend, is de isolatie van pulmonale endotheelcellen essentieel voor studies naar longontsteking. Dit protocol beschrijft een procedure voor de hoogproductieve, zeer zuivere isolatie van macrovasculaire en microvasculaire endotheelcellen.

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Deze maand in JoVE nummer 192 Endotheelcellen explant endotheel longen celisolatie ontsteking
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