Crispr Engineered Cells
Crispr Engineered Cells - Related Videos
06:12
Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
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Cited by 24 •
2018
Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.
06:33
Production of Human CRISPR-Engineered CAR-T Cells
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Cited by 19 •
2021
Here, we present a protocol for gene editing in primary human T cells using CRISPR Cas Technology to modify CAR-T cells.
08:20
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
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Cited by 13 •
2020
Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell therapeutics.
02:55
Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
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2025
The video outlines a process for creating genetically modified CAR T cells through the CRISPR-Cas9 System. Infecting T cells with CRISPR and CAR lentiviruses results in modifications to the target gene and the synthesis of a chimeric antigen receptor or CAR, ultimately leading to the formation of genetically modified CAR T cells.
21:08
Engineering Cell-permeable Protein
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Cited by 12 •
2009
Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.
FAQs
The guide RNA supplies the sequence-matching information that positions a CRISPR-associated nuclease at a chosen DNA site. Cas9, for example, then creates the targeted break rather than acting throughout the genome indiscriminately. This division of roles matters in bioengineering because sequence recognition determines where a cellular function can be perturbed or redesigned for investigation.
The repair route determines how a CRISPR-induced break is resolved. End joining repairs the chromosome by reconnecting the broken ends, whereas template-directed repair uses a repair template to guide restoration at the targeted region. Distinguishing these pathways is important when engineered cells are created to investigate gene function or redesign a cellular process.
Precise targeting links a deliberate DNA change to a specific cellular question. When the selected sequence lies in a gene or pathway of interest, the resulting engineered cell can help investigators examine how that function operates or how changing it alters a cellular system. This connection supports both mechanistic studies and redesign of cells with defined properties.
A basic workflow begins by selecting a complementary DNA sequence and using it to specify a guide RNA. The guide RNA directs a CRISPR-associated nuclease, such as Cas9, to that site, where the nuclease makes a targeted break. The cell then repairs the break through end joining or template-directed repair, producing the engineered cellular state.
Researchers can use these cells to study gene function, disease mechanisms, or cellular pathways. The same platform supports therapeutic development and biomanufacturing, where engineered cellular properties can be examined in a defined context. It also contributes to regenerative medicine and cell-based systems designed with specified characteristics, connecting genetic intervention with practical bioengineering goals.
By changing selected genetic material, investigators can connect a defined DNA intervention with the behavior or function of a cell. That connection allows bioengineers to investigate cellular pathways while also redesigning cells for defined properties. Consequently, engineered cells can serve as research systems and as components of broader efforts in regenerative medicine, therapeutic development, and biomanufacturing.