Ex Vivo Lung Specimens
Ex Vivo Lung Specimens - Related Videos
05:55
Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging
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2023
This video describes a method of preparing lungs for ex vivo live imaging to study interactions between cancer cells and stromal cells, using agarose for inflation. In the sample protocol, agarose inflation procedure is described before observing live cellular interactions.
11:19
Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
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Cited by 6 •
2016
We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
14:21
Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation
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Cited by 21 •
2013
A method to image ex vivo pulmonary resection specimens with optical frequency domain imaging (OFDI) and obtain precise correlation to histology is described, which is essential to developing specific OFDI interpretation criteria for pulmonary pathology. This method is applicable to other tissue types and imaging techniques to obtain precise imaging to histology correlation for accurate image interpretation and assessment. Imaging criteria established with this technique would then be...
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
FAQs
These conditions help preserve the structural and functional relevance of the preparation outside the body. Perfusion supports the vascular component, ventilation maintains airway-related mechanical conditions, and controlled temperature and nutrient supply help sustain the tissue. Maintaining this combination allows researchers to examine transport, injury, repair, and mechanical responses under controlled experimental conditions.
Native architecture retains relationships among cells, extracellular matrix, airways, and blood vessels that simplified systems may not reproduce. Those relationships influence how materials, drugs, or engineered grafts interact with lung tissue. Consequently, measurements made in preserved specimens can provide more biologically relevant evidence for refining regenerative technologies and other bioengineering designs.
They occupy an intermediate experimental space. Compared with simpler in vitro models, these preparations retain native tissue organization; compared with whole-animal experiments, they reduce biological complexity and permit more direct control and measurement. This position helps researchers evaluate designs and responses before advancing toward in vivo studies, while still recognizing that the preparation remains viable only for a limited period.
Researchers can directly assess transport, injury, repair, and mechanical responses while the relevant tissue structures remain accessible. These measurements connect physical conditions or engineered interventions with effects on airways, vessels, cells, and extracellular matrix. The resulting observations can reveal how a biomaterial, delivery system, or graft design performs within native lung architecture rather than in an isolated simplified system.
A typical workflow begins with a lung or lung section, followed by establishment of suitable perfusion and, when required, ventilation conditions. Researchers then control temperature and nutrient availability to preserve the preparation for a limited period. Once maintained, the specimen can be used for direct measurements or evaluation of a selected bioengineering intervention.
They are useful when investigators need controlled access to native lung tissue while testing biomaterials, tissue-engineered grafts, imaging methods, or drug-delivery systems. The preparation supports direct observation and measurement without the full complexity of a whole-animal experiment. It is therefore especially relevant during development and evaluation of regenerative lung technologies before in vivo investigation.
They provide a testing context in which engineered materials or grafts can be examined alongside native cells, extracellular matrix, airways, and blood vessels. Researchers can evaluate transport, mechanical behavior, injury, and repair under controlled conditions. Findings from this intermediate stage can guide design decisions and help connect in vitro development with later in vivo studies.