Coronary Perfusion
Coronary Perfusion - Related Videos
11:30
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
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Cited by 68 •
2012
A method to rapidly and completely remove cellular components from an intact porcine heart through retrograde perfusion is described. This method yields a site specific cardiac extracellular matrix scaffold which has the potential for use in multiple clinical applications.
10:16
Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
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Cited by 25 •
2012
Visualization of the coronary vessels is critical to advancing our understanding of cardiovascular diseases. Here we describe a method for perfusing murine coronary vasculature with a radiopaque silicone rubber (Microfil), in preparation for micro-Computed Tomography (μCT) imaging.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
06:25
Coronary Angiography During Ex-Situ Heart Perfusion in a Porcine Model
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2024
Screening for coronary artery disease could increase the procurement of donor hearts with extended criteria. A protocol for performing reproducible coronary angiography during ex-situ heart perfusion is described herein in a porcine model.
03:44
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
FAQs
Diastole is critical because ventricular relaxation reduces the compression placed on blood vessels running through the heart muscle. This creates a more favorable period for coronary blood flow than ventricular contraction, when intramyocardial vessels are compressed. The timing links the cardiac cycle to myocardial oxygen delivery and helps explain why changes in relaxation can affect perfusion.
Heart rate affects coronary perfusion by changing the duration of each cardiac-cycle phase. When the rate rises, the available time for ventricular relaxation and coronary blood flow may become shorter, while myocardial work and oxygen demand increase. Studying this relationship helps explain how cardiac timing can influence the balance between oxygen supply and demand.
Local metabolic signals adjust coronary vessel diameter in response to the heart muscle’s activity and oxygen requirements. Increased myocardial demand promotes changes that help align blood delivery with tissue needs, whereas lower demand requires less flow. This local regulation allows coronary circulation to respond directly to the functional state of the myocardium.
Blood pressure provides the driving force for coronary blood flow, while vascular resistance influences how readily blood passes through the coronary circulation. Changes in either variable can alter the amount of oxygen-rich blood reaching the myocardium. Considering both factors helps researchers interpret how cardiovascular conditions may disturb perfusion and cardiac performance.
Researchers can examine how coronary perfusion changes alongside heart rate, blood pressure, vascular resistance, and cardiac function. These comparisons reveal whether blood delivery remains matched to myocardial activity and oxygen demand. The resulting physiological context is useful for interpreting altered circulation and for explaining why impaired perfusion can compromise continuous cardiac contraction.
Coronary perfusion provides a physiological framework for understanding myocardial ischemia, coronary artery disease, and infarction. When blood delivery fails to meet myocardial requirements, oxygen availability becomes inadequate for the heart muscle. Investigating perfusion therefore supports interpretation of disease-related changes and underlies diagnostic techniques designed to assess blood flow to the heart.