Metabolism Screening
Metabolism Screening - Related Videos
06:50
Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening
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Cited by 1 •
2025
Here, we establish a metabolic profiling platform integrating three-dimensional (3D) glioma spheroids and imaging-based normalization via extracellular flux analysis. This approach enables more accurate assessment of tumor metabolic responses to drug treatments and provides insights into potential mechanisms of resistance.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
03:01
alamarBlue Assay: A Sensitive Fluorometric Method to Screen the Cytotoxic Effects of Artificial Tear Formulations on Metabolic Activity of Human Corneal Epithelial Cells
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2025
This video describes an in vitro fluorometric assay to assess the effects of artificial tear formulations on the cellular metabolic activity of corneal epithelial cells using resazurin-based Alamar blue solution. A non-toxic formulation maintains the cells in a healthy, metabolically active state, facilitating the reduction of resazurin into a highly fluorescent resorufin.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
Substrate consumption shows which nutrients or compounds cells, tissues, or organisms use, while product formation indicates what those inputs become. Examining both measurements helps distinguish reduced uptake from altered downstream processing. Under defined conditions, changes in these values can reveal whether an experimental treatment affects a particular biochemical transformation, overall energy use, or cellular growth.
Metabolic flux describes the movement of materials through biochemical pathways over time. Screening can use changes in flux to connect individual reactions with broader pathway behavior rather than relying on a single measurement. This perspective helps researchers determine whether an observed response reflects pathway reorganization, altered nutrient handling, or a change in the balance between consumption and product formation.
Defined conditions make measurements more comparable by limiting differences unrelated to the experimental question. Comparing normal samples with experimentally treated samples then provides a reference for identifying treatment-associated changes in substrate use, products, enzyme activity, or flux. This design helps separate an altered metabolic response from the baseline behavior of the biological system.
Enzyme activity provides a functional view of the biochemical reactions that support metabolic changes. When activity shifts alongside substrate consumption or product formation, the results can link an observable metabolic pattern to altered reaction capacity. This connection is useful for characterizing cellular responses and for determining whether a treatment may influence a pathway at the enzymatic level.
A basic workflow establishes defined experimental conditions, selects normal and experimentally treated samples when comparison is needed, and measures relevant indicators such as substrate consumption, product formation, enzyme activity, or metabolic flux. Researchers then compare the resulting patterns and relate them to growth, energy use, or cellular behavior. The measurements chosen should match the biological question.
The approach is useful when a researcher needs to determine whether a drug or toxin changes how cells, tissues, or organisms process nutrients and other compounds. It can also identify metabolic signatures associated with disease. Comparing treated or disease-related samples with normal biological material may reveal pathway alterations that are not apparent from growth or behavior alone.
Metabolism screening connects biochemical reactions with measurable biological outcomes, making it relevant across physiology, microbiology, pharmacology, and biotechnology. In physiology, it can clarify energy use; in microbiology, it can characterize cellular responses; in pharmacology, it can assess treatment effects; and in biotechnology, it can help evaluate metabolic behavior under experimentally selected conditions.