Pipette Access
Pipette Access - Related Videos
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
14:37
Accessibility to Clinical Care for People who use Wheelchairs
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2023
Source: Yetsa Tuakli-Wosornu1,2, Jaideep Talwalkar1; 1Yale School of Medicine, 2University of Pittsburgh In the United States, 25% of the general population suffers from one or another type of disability. Ambulatory disabilities, or mobility impairments, represent the most common subcategory, comprising 14% of the country's populace. Different mobility-assistive devices, ranging from canes to scooters, enable increased independence and improved quality of life for those suffering from mobility...
05:12
Pressure-polishing Pipettes for Improved Patch-clamp Recording
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Cited by 13 •
2008
This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
FAQs
Microscopic control makes pipette access a precision process rather than a simple insertion. The operator aligns the fine glass pipette with the selected cell and adjusts gentle pressure or suction to establish contact or entry. This controlled approach helps limit physical damage, which is important when measurements or material delivery depend on preserving the cell during the experiment.
Membrane penetration allows the pipette to reach the cell interior rather than interact only with fluid outside the cell. That distinction supports intracellular recording and examination of cellular contents, while contact with surrounding fluid can support sampling or other external interactions. The selected form of access therefore determines which cellular compartment can be studied.
Pipette access works at the level of individual cells, giving researchers localized control over measurement, sampling, delivery, or manipulation. Bulk methods combine signals or materials from many cells, whereas this approach can focus on one selected cell. That single-cell precision is especially valuable for investigating electrical activity and cellular contents that may be obscured in larger samples.
These actions provide different ways to establish interaction with a cell or its surrounding fluid. Gentle pressure can assist delivery, suction can support access or fluid sampling, and membrane penetration can provide entry for intracellular work. Researchers select among them according to whether the experiment requires recording, microinjection, sampling, or another form of individual-cell manipulation.
The essential setup combines a fine glass micropipette with microscopic positioning and controlled application of pressure or suction. The microscope guides alignment with the target cell, while the pipette provides the narrow path for contact, entry, sampling, or delivery. Maintaining gentle control is central because the method aims to obtain access while limiting physical damage.
This approach supports intracellular recording, microinjection, fluid sampling, and manipulation of individual cells. It can therefore connect electrical measurements with direct interaction with cellular contents or nearby fluid. In biology, these capabilities help researchers examine cell-specific behavior and processes that cannot be adequately resolved by methods treating a larger population as a single sample.