Progranulin Bait
Progranulin Bait - Related Videos
07:56
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
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Cited by 14 •
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We have modified the conventional yeast two-hybrid screening, an effective genetic tool in identifying protein interaction. This modification markedly shortens the process, reduces the workload, and most importantly, reduces the number of false positives. In addition, this approach is reproducible and reliable.
03:55
ELISA-Based Method to Analyze Interactions between Bait and Prey Proteins
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This video describes an ELISA-based technique that detects protein-protein interactions between two proteins by measuring the binding of the second protein to the immobilized first protein. The bound second protein is detected using specific antibodies labeled with an enzyme, such as horseradish peroxidase.
12:36
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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Phage display is a powerful technique to capture proteins or protein moieties that interact with an immobilized molecule of interest. Once a decision of the type of phage cDNA library to create and screen has been made, the protocol described here permits efficient affinity selection leading to identification of interactors.
07:58
Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis
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We describe a protocol for the destabilization of the medial meniscus (DMM) model in mice, an effective tool for osteoarthritis (OA) research. In addition, we have demonstrated that deficiency of progranulincan exaggerate OA development and progression by using this model, indicating that progranulin plays a protective role in the pathogenesis of OA.
03:34
Assessing Social Dominance in Mouse Models Using the Tube Test
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Cited by 1 •
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This protocol describes a behavioral assay to evaluate social dominance in rodents using the tube test. Social dominance remains stable over time, and several models of developmental and neurological disorders exhibit robust social dominance abnormalities. Therefore, the tube test serves as a convenient outcome measure for mechanistic studies or preclinical therapeutic screening.
FAQs
Affinity retention is the central mechanism. In a Progranulin Bait assay, molecules that associate with the bait remain associated with the solid support during washing, while other components are removed. Elution then recovers the retained material for analysis. This enrichment helps separate progranulin-associated proteins, receptors, and regulatory factors from the original mixture.
Tagging and immobilization provide two ways to present progranulin as bait. In one format, the protein is attached to a solid support; in another, it is expressed as a bait construct. This choice determines how progranulin is introduced to a protein mixture or cell lysate and can influence how interaction partners are captured for downstream analysis.
Washing follows incubation and separates retained molecules from components that did not remain associated with the bait. This step is essential for producing an eluate enriched in progranulin-associated material rather than the entire starting mixture. The resulting sample provides a more focused basis for analyzing proteins, receptors, or regulatory factors linked to progranulin.
The main readout is the set of molecules recovered after washing and elution. Analysis of that material can reveal progranulin-associated proteins, receptors, and regulatory factors, allowing researchers to examine candidate interaction networks. Because the bait can be incubated with a protein mixture or cell lysate, the results place progranulin associations within a broader cellular molecular context.
Within biology, the method can support studies of signaling, trafficking, and lysosomal biology through the molecules captured with progranulin. These applications connect a biochemical affinity assay with questions about cellular pathways and compartments. Examining the recovered associations can therefore help researchers investigate how progranulin-related interactions fit into broader biological systems.
Identifying molecules associated with progranulin can support investigation of progranulin-related disorders. The captured proteins, receptors, and regulatory factors provide molecular information that may help researchers examine interaction networks and the mechanisms underlying disease. This makes the assay useful for connecting progranulin binding behavior with disease-focused studies of signaling, trafficking, or lysosomal biology.