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Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
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Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Article DOI: 10.3791/3562 • 07:57 min • January 17th, 2012
January 17th, 2012 •
1, 1, 1,2
1Department of Orthopaedic Surgery, NYU Hospital for Joint Diseases, 2Department of Cell Biology, New York University School of Medicine

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Summary
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We have modified the conventional yeast two-hybrid screening, an effective genetic tool in identifying protein interaction. This modification markedly shortens the process, reduces the workload, and most importantly, reduces the number of false positives. In addition, this approach is reproducible and reliable.

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Modified Yeast-two-hybrid System Proteins Interacting Growth Factor Progranulin PGRN Granulin Epithelin Precursor Autocrine Growth Factor Physiologic Processes Disease Processes Inflammation Host Defense Neurotrophic Factor PGRN Gene Frontotemporal Dementia Cartilage Development And Degradation Binding Receptor(s) Modified Yeast Two-hybrid Approach GAL4 Based 2-hybrid System False Positive Clones Reproducible And Reliable System
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