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Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
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Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

English

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28,513 Views

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07:56 min

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January 17, 2012

DOI:

10.3791/3562-v

DOI:
10.3791/3562-v

•

07:56 min
January 17, 2012

•

5 Views
, ,
1Department of Orthopaedic Surgery,NYU Hospital for Joint Diseases, 2Department of Cell Biology,New York University School of Medicine

Summary

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We have modified the conventional yeast two-hybrid screening, an effective genetic tool in identifying protein interaction. This modification markedly shortens the process, reduces the workload, and most importantly, reduces the number of false positives. In addition, this approach is reproducible and reliable.

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Cite this Article

Tian, Q., Zhao, Y., Liu, C. Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin. J. Vis. Exp. (59), e3562, doi:10.3791/3562 (2012).

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