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JoVE Journal Biology
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
 

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Article doi: 10.3791/3562
January 17th, 2012 •
1, 1, 1,2
1Department of Orthopaedic Surgery, NYU Hospital for Joint Diseases, 2Department of Cell Biology, New York University School of Medicine

Summary
Automatic Translation
January 17th, 2012

We have modified the conventional yeast two-hybrid screening, an effective genetic tool in identifying protein interaction. This modification markedly shortens the process, reduces the workload, and most importantly, reduces the number of false positives. In addition, this approach is reproducible and reliable.

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