Xenogen Ivis
Xenogen Ivis - Related Videos
09:01
A Quantitative Assessment of the Phagocytosis of Allogeneic and Xenogeneic Erythrocytes by Rat Macrophages In Vitro
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2025
This work describes a standardized in vitro protocol to quantify and compare the phagocytosis rates of xenogeneic (human) and allogeneic (rat) red blood cells by isolated rat macrophages.
08:53
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
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Cited by 57 •
2009
Mammary tumor cells expressing luciferase are implanted subcutaneously in mice and visualized using optical imaging to monitor tumor growth and development non-invasively in a longitudinal study.
10:21
In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
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Cited by 11 •
2012
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over...
06:00
Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy
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Cited by 3 •
2023
Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.
06:06
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
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Cited by 1 •
2019
Here, we present a protocol to induce and score disease in a xenogeneic graft-versus-host disease (xenoGVHD) model. xenoGVHD provides an in vivo model to study immunosuppression of human T cells. Additionally, we describe how to detect human T cells in tissues with digital PCR as a tool to quantify immunosuppression.
FAQs
A sensitive camera detects photons emitted from bioluminescent or fluorescent markers in a living organism. The system converts those detected signals into images showing where reporter activity occurs and how it is distributed spatially. Researchers can therefore relate signal patterns to the location of biological events, such as gene expression or the presence of labeled cells, within the animal model.
Luciferase reporter genes provide a way to associate emitted light with activity from a selected biological system. When researchers introduce these genes into an experimental model, the resulting signal can be detected and mapped by IVIS. This makes reporter activity measurable in living subjects and supports studies that track changes in gene expression or other processes over time.
IVIS can visualize signals produced by luciferase-based reporter genes as well as signals from fluorescent markers. These two marker types provide alternative ways to label or report biological activity, while the imaging system records their emitted signals and displays their spatial distribution. The selected signal type determines how the biological feature of interest is represented in the resulting images.
Repeated imaging allows the same subjects to be assessed at multiple time points rather than relying only on terminal sampling. Researchers can follow disease progression, tumor growth, infection, cell trafficking, or treatment effects within individual animals. This longitudinal design reveals changes over time and reduces the need to end separate subjects for each measurement.
A typical workflow begins by introducing a luciferase-based reporter gene or fluorescent marker into the biological model. Researchers then place the living subject within the imaging system, where a sensitive camera detects the emitted signal. The instrument converts those measurements into images that can be examined for reporter activity and its spatial distribution.
The approach supports a broad range of biological investigations, including gene expression, tumor growth, infection, cell trafficking, and therapeutic responses. Its value comes from connecting visible reporter signals with processes occurring in living subjects. Because measurements can be repeated, researchers can compare how these processes develop and how they change during treatment or disease progression.