Rhodamine 123
Rhodamine 123 - Related Videos
09:14
Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
0 Views •
Cited by 10 •
2011
A method to visualize and quantify F-actin barbed ends in neuronal growth cones is described. After culturing neurons on glass coverslips, cells are permeabilized with a saponin-containing solution. Then, a short incubation with the saponin buffer containing rhodamine-actin incorporates fluorescent actin onto free actin barbed ends.
07:10
Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes
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Cited by 7 •
2021
Here, we present a protocol for studying mating competitiveness of male Aedes aegypti using fluorescent dye as a marker. Female mosquitoes are exposed to both marked and unmarked males for copulation. Post mating, their spermathecae are examined under a fluorescence microscope to determine their mating partner.
02:53
Functionalized Nanoparticle Targeting of Ovarian Cancer: A Technique to Facilitate Folic Acid-conjugated Nanoparticle Contrast Agent Uptake by Ovarian Cancer Cells
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2023
In this video, we demonstrate a technique to facilitate the internalization of folic acid-capped copper sulfide nanoparticles (CuS NPs). Folic acid-functionalized CuS NPs have the ability to target folate-receptors which are overexpressed in many ovarian cancer cells.
09:05
Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
0 Views •
Cited by 9 •
2015
Nitrogen is an effective supercritical fluid for extraction or drying processes due to its small molecular size, high density in the near-liquid supercritical regime, and chemical inertness. We present a supercritical nitrogen drying protocol for the purification treatment of reactive, porous materials.
10:41
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
0 Views •
Cited by 2 •
2017
A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.
FAQs
The inner mitochondrial membrane potential determines how much Rhodamine 123 accumulates inside mitochondria. A stronger potential favors greater accumulation of this positively charged dye, whereas loss of polarization reduces that driving force. Consequently, fluorescence measurements can be interpreted as indicators of mitochondrial polarization status, provided comparisons are made under consistent experimental conditions.
Transporter-mediated efflux can reduce intracellular Rhodamine 123 independently of changes in mitochondrial polarization. Lower fluorescence may therefore reflect dye removal as well as altered mitochondrial function. This distinction makes the dye useful for investigating multidrug resistance, but it also means that fluorescence differences should be interpreted alongside the possibility of transporter activity.
Changes in Rhodamine 123 fluorescence can signal altered mitochondrial polarization, depolarization, or cellular injury. In cancer research, these shifts help investigators monitor mitochondrial changes associated with apoptosis-related processes. The readout is therefore valuable for detecting treatment-associated cellular responses, although fluorescence changes should be interpreted in relation to the experimental treatment and cell population being examined.
A typical workflow applies Rhodamine 123 staining to the cancer or comparison cell population, then measures fluorescence using microscopy or flow cytometry. Researchers can compare tumor and normal cells, or examine treated and untreated conditions, to identify differences in mitochondrial activity. The resulting fluorescence patterns provide a basis for evaluating polarization changes and treatment responses.
Fluorescence microscopy and flow cytometry provide complementary ways to assess Rhodamine 123 staining. Microscopy supports visualization of mitochondrial fluorescence within cells, whereas flow cytometry enables fluorescence-based comparison across analyzed cell populations. Selecting between them depends on whether the study emphasizes cellular visualization or comparative measurement of mitochondrial activity among different samples.
Researchers use Rhodamine 123 to examine how cancer cells respond to drug treatment through changes in mitochondrial fluorescence. The assay can help identify treatment-associated depolarization or cellular injury and can also reveal the influence of transporter-mediated efflux. These measurements connect mitochondrial behavior with drug mechanisms and provide context for investigating multidrug resistance in tumor cells.