Lc-ms
Lc-ms - Related Videos
11:16
Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
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Cited by 11 •
2014
HaloTag technology is a multifunctional technology which has shown significant success in isolation of both small and large protein complexes from mammalian cells. Here we highlight the advantages of this technology compared to existing alternatives and demonstrate its utility to study numerous aspects of protein function inside eukaryotic cells.
10:49
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
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Cited by 60 •
2013
A bioprinter was used to create patterned hydrogels based on a sacrificial mold. The poloxamer mold was backfilled with a second hydrogel and then eluted, leaving voids which were filled with a third hydrogel. This method uses fast elution and good printability of poloxamer to generate complex architectures from biopolymers.
06:49
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
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Cited by 8 •
2018
This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software.
10:22
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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Cited by 14 •
2011
Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.
12:50
Lesion Explorer: A Video-guided, Standardized Protocol for Accurate and Reliable MRI-derived Volumetrics in Alzheimer's Disease and Normal Elderly
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Cited by 29 •
2014
Lesion Explorer (LE) is a semi-automatic, image-processing pipeline developed to obtain regional brain tissue and subcortical hyperintensity lesion volumetrics from structural MRI of Alzheimer's disease and normal elderly. To ensure a high level of accuracy and reliability, the following is a video-guided, standardized protocol for LE's manual procedures.
FAQs
Within the liquid-chromatography stage, compounds do not all travel through the column at the same rate. Their polarity and interactions with the stationary phase influence when they elute. This timing separates components before mass analysis, helping distinguish substances in complex mixtures and allowing the subsequent measurements to be associated with individual chromatographic components.
After compounds leave the column, they are ionized and sorted according to their mass-to-charge ratios. The resulting characteristic spectra provide molecular-mass information and can also contribute structural information. This mass-based pattern complements chromatographic retention differences, giving analysts more than one basis for recognizing or characterizing components in a sample.
The two stages address different analytical challenges. Chromatography separates components that occur together, while mass-based detection distinguishes the resulting ions by their mass-to-charge ratios. Their combination improves selectivity, meaning the ability to focus on particular components, and supports sensitive detection of trace compounds that could be difficult to assess in an unresolved mixture.
A typical workflow begins when a liquid mobile phase carries the sample through a chromatographic column. Separated compounds elute at different points, then enter the mass-analysis stage, where they are ionized and sorted by mass-to-charge ratio. Analysts can interpret the chromatographic separation together with the resulting spectra to identify and measure mixture components.
LC-MS is especially useful when a sample contains multiple compounds, trace components, or substances requiring molecular characterization. Its combined separation and detection capabilities support pharmaceutical analysis, metabolite profiling, environmental testing, and reaction monitoring. Depending on the investigation, the data can reveal component presence, relative analytical response, molecular mass, or structural clues.
In pharmaceutical analysis, the method helps examine compounds and related components; metabolite profiling uses it to investigate mixtures of metabolites; environmental testing benefits from trace-compound detection. During reaction monitoring, chromatographic changes and mass-based information can help follow chemical components over the course of an investigation, while spectra assist characterization of unknown substances.