Solution-phase Nmr
Solution-phase Nmr - Related Videos
16:20
Hyperpolarized Xenon for NMR and MRI Applications
0 Views •
Cited by 28 •
2012
The production of hyperpolarized xenon by means of spin exchange optical pumping (SEOP) is described. This method yields a ~10000-fold enhancement of the nuclear spin polarization of Xe-129 and has applications in nuclear magnetic resonance spectroscopy and imaging. Examples of gas phase and solution state experiments are given.
11:44
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
0 Views •
Cited by 4 •
2016
A detailed protocol describing the SSTD NMR method is presented here to help new users apply this new method to obtain the kinetic parameters of their own systems undergoing chemical exchange.
10:08
Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
0 Views •
Cited by 20 •
2017
Experiments on phase separated giant unilamellar vesicles (GUVs) frequently neglect physiological solution conditions. This work presents approaches to study the effect of high-salinity buffer on liquid-liquid phase separation in charged multicomponent GUVs as a function of trans-membrane solution asymmetry and temperature.
09:24
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
0 Views •
Cited by 2 •
2017
A simple and non-destructive technique that measures the average content of drug substances in formulated drug products containing fluorine using low-field fluorine-19 (19F) time-domain (TD) nuclear magnetic resonance (NMR) is presented here. The technique can be applied to the development and manufacturing of drugs in the pharmaceutical industry.
10:08
Nuclear Magnetic Resonance (NMR) Spectroscopy
0 Views •
2023
Source: Laboratory of Dr. Henrik Sundén – Chalmers University of Technology Nuclear magnetic resonance (NMR) spectroscopy is a vital analysis technique for organic chemists. With the help of NMR, the work in the organic lab has been facilitated tremendously. Not only can it provide information about the structure of a molecule but also determine the content and purity of a sample. Compared with other commonly encountered techniques for organic chemists — such as thermal analysis and mass...
FAQs
Chemical shifts reflect the local electronic environments surrounding observed nuclei. Nuclei in different molecular settings therefore produce signals at different spectral positions, allowing researchers to compare environments within a compound. Interpreting these shifts helps identify compounds and contributes to determining molecular structure rather than merely detecting that a sample contains nuclei.
Spin-spin couplings provide information about nuclear connectivity, while signal intensities indicate the relative contribution of detected nuclei to the spectrum. Together with chemical shifts, these features give complementary evidence about how atoms are related within a molecule. Researchers use the combined pattern to support compound identification and structural analysis.
The instrument detects an NMR response in the time domain after radiofrequency excitation. Fourier transformation converts that time-domain signal into a spectrum containing interpretable signal positions and intensities. This conversion makes chemical shifts, spin-spin couplings, and signal patterns accessible for structural and analytical interpretation.
Because measurements occur with molecules dissolved in a liquid, solution-phase NMR can examine conformational studies and molecular dynamics under near-native conditions. Changes or patterns in the observed signals can be interpreted in relation to molecular behavior. This makes the method useful when researchers need information beyond a single static structural description.
Researchers place the molecule in a liquid solution, expose its nuclei to a strong magnetic field, and apply radiofrequency pulses. The resulting emitted signal is detected in the time domain, transformed into a spectrum, and interpreted using chemical shifts, couplings, and intensities. This workflow supports compound identification and molecular-structure determination.
The method supports several analytical and research tasks, including mixture analysis, purity assessment, reaction monitoring, conformational studies, and investigations of molecular dynamics. Its value depends on the information required: spectra can help identify compounds, evaluate sample composition, follow chemical changes, or examine behavior of dissolved molecules under near-native conditions.