Surface Functionalization Identification

Surface functionalization identification is the analytical process of determining which chemical groups or molecular layers have been introduced onto a material’s surface and how they are distributed. It combines techniques such as X-ray photoelectron spectroscopy, infrared spectroscopy, contact-angle measurements, and microscopy to relate surface composition and wettability to specific chemical modifications. In chemistry, this analysis helps verify reactions including silanization, polymer grafting, oxidation, and biomolecule immobilization without relying solely on bulk composition. Reliable identification supports the design and quality control of catalysts, sensors, coatings, membranes, nanoparticles, and biomaterials by linking interfacial chemistry to performance.

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Research

JoVE Journal - Genetics
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Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

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Cited by 8 •

2020

This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.

Research

JoVE Journal - Immunology and Infection

Peptide-based Identification of Functional Motifs and their Binding Partners

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Cited by 2 •

2013

Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.

A Method of Targeted Cell Isolation via Glass Surface Functionalization

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Cited by 6 •

2016

This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

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Cited by 15 •

2018

We have engineered the capsid protein of hepatitis E virus as a theranostic nanoparticle (HEVNP). HEVNP self-assembles into a stable icosahedral cage in mucosal delivery. Here, we describe the modification of HEVNPs for tumor targeting by mutating surface-exposed residues to cysteines, which conjugate synthetic ligands that specifically bind tumor cells.

Combined Invasive Subcortical and Non-invasive Surface Neurophysiological Recordings for the Assessment of Cognitive and Emotional Functions in Humans

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Cited by 5 •

2016

The present protocol aims at assessing cognitive-emotional functions in the basal ganglia by simultaneous neurophysiological recording of local field potentials and non-invasive brain cortical activity (EEG). The procedure is exemplified by the use of paradigms involving speech stimuli with emotional connotation or the Flanker task involving cognitive control.

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