Desorption Efficiency
Desorption Efficiency - Related Videos
07:12
An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
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Cited by 3 •
2018
A protocol for enhancing carbohydrate ion signals in MALDI mass spectrometry by reforming crystalline structures during sample preparation processes is demonstrated.
10:47
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Cited by 19 •
2016
A mass spectrometry imaging (MSI) source operated at atmospheric pressure was developed by coupling mid-infrared laser desorption and electrospray post-ionization. Exogenous ice matrix was used as the energy-absorbing matrix to facilitate resonant desorption of tissue-related material. This manuscript provides a step-by-step protocol for performing IR-MALDESI MSI of whole-body neonatal mouse.
01:08
Matrix-Assisted Laser Desorption Ionization (MALDI)
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2025
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
06:21
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Cited by 21 •
2013
Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient method by which samples, including biological tissues, can be imaged with minimal sample preparation. By rastering the sample below the ionization probe, this spray-based technique provides sufficient spatial resolution to discern molecular features of interest within tissue sections.
09:38
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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Cited by 3 •
2013
Dithranol (DT; 1,8-dihydroxy-9,10-dihydroanthracen-9-one) has previously been reported as a MALDI matrix for tissue imaging of small molecules; protocols for the use of DT for the MALDI imaging of endogenous lipids on the surface of tissue sections by positive-ion MALDI-MS on an ultrahigh-resolution quadrupole-FTICR instrument are provided here.
FAQs
The strength of analyte binding to a sampling medium sets the difficulty of releasing the compound. Solvent composition, temperature, contact time, and flow then influence how much is recovered. These variables act together: insufficient release lowers recovery, while unsuitable conditions can contribute to degradation or loss. Controlling them is therefore central to obtaining dependable environmental measurements.
Desorption efficiency matters because a measured concentration can be biased when the extraction step does not recover the compound consistently. Laboratories can use the measured efficiency to correct environmental sample results, rather than treating the recovered amount as fully representative of what was present on the sampling medium. Consistency across analyses also strengthens confidence in comparisons and decisions.
Optimization requires balancing release against preservation of the analyte. Changes to solvent composition, temperature, contact time, or flow may improve recovery, but conditions must release the compound without causing degradation or loss. Consequently, a condition that produces a larger recovered amount is not automatically preferable if it compromises analyte integrity or reproducibility.
Laboratories evaluate desorption efficiency by examining the proportion of analyte recovered from the sampling medium during the extraction or desorption step. The assessment should document relevant conditions, including solvent composition, temperature, contact time, and flow, because these variables affect release. The resulting efficiency can support correction of environmental sample results and evaluation of method performance.
Desorption efficiency is relevant to air, soil, water, and sediment analysis, but the sampling medium and analyte-medium interaction determine how readily release occurs. Evaluating recovery within each analytical context helps laboratories judge whether a method performs reliably for that sample type. This is important when results are used to characterize pollutants across different environmental media.
High, consistent desorption efficiency improves confidence that reported pollutant measurements reflect the sampled material rather than avoidable extraction losses. That reliability supports exposure assessment and regulatory decision-making, where inaccurate or inconsistent results could weaken interpretation. The metric also helps laboratories evaluate analytical methods, linking a technical extraction characteristic to broader environmental monitoring conclusions.