Functional Genomics Screening
Functional Genomics Screening - Related Videos
11:59
Competitive Genomic Screens of Barcoded Yeast Libraries
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Cited by 44 •
2011
We have developed comprehensive, unbiased genome-wide screens to understand gene-drug and gene-environment interactions. Methods for screening these mutant collections are presented.
10:58
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
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Cited by 25 •
2012
We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.
08:40
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Cited by 1 •
2012
The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.
03:48
Screening for Genomic Modifications: A Method to Identify CRISPR-Generated Mutants in Drosophila
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2023
This video describes a screening method to identify transgenic Drosophila flies, whose genome was modified using CRISPR-Cas9. CRISPR-Cas9 is a powerful genome-editing tool that revolutionized the way scientists can manipulate an organism's genome. In the example protocol, we will see how to identify CRISPR-generated mutants, in which an insertion of a transactivation sequence replaces the first exon of the branchless (bnl) gene, thus creating a bnl gene-specific driver line, bnl-LexA.
11:30
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
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Cited by 8 •
2017
Bacterial effector proteins are important for establishing successful infections. This protocol describes the experimental identification of proteinaceous binding partners of a bacterial effector protein in its natural plant host. Identifying these effector interactions via yeast two-hybrid screens has become an important tool in unravelling molecular pathogenicity strategies.
FAQs
Different perturbation types let a screen test gene function from complementary directions. CRISPR-mediated knockout, activation, and RNA interference can be selected as targeted ways to alter gene activity, while the resulting cellular changes reveal whether a gene contributes to a measured trait. This comparison helps connect a genetic perturbation to its observable phenotype.
Control cells provide the reference needed to interpret changes caused by a perturbation. Researchers compare perturbed and control populations through phenotypic measurements or sequencing-based readouts, helping distinguish changes associated with the targeted genetic intervention from the background state of the cells. This comparison is central to assigning a phenotype to a gene or regulatory element.
Phenotypic assays capture changes in cellular traits, whereas sequencing-based readouts provide another measurement of screen results. Functional Genomics Screening can use either type of readout to evaluate how perturbations affect cells. Considering the readout alongside the experimental comparison helps investigators relate observed cellular changes to the genes or regulatory elements being tested.
An experiment typically begins by introducing a selected perturbation into a cell population, alongside control cells. Investigators then measure resulting changes with a phenotypic assay or sequencing-based readout and compare the groups. The final interpretation links observed differences to genes or regulatory elements and to the cellular process under study.
These screens are useful when researchers need to identify genes involved in pathways, disease mechanisms, drug responses, or broader cellular processes. They can also support target discovery and disease modeling. In each case, the value comes from relating a deliberate genomic perturbation to a measurable cellular change rather than relying only on sequence information.
Sequence analysis can identify genomic variants, but it does not by itself establish whether a variant has a biological effect. Functional genomics screening adds an experimental test by perturbing relevant genes or regulatory elements and observing cellular outcomes. This makes the approach useful for connecting genetic variation with phenotype in disease and therapeutic research.