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Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

DOI:
10.3791/55150-v

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11:30 min

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January 20, 2017

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,
1Department of Molecular Biology – Functional Genomics,Laimburg Research Centre

Chapters

  • 00:05Title
  • 00:48Collecting Root and Leaf Samples from Infected Apple Trees
  • 02:22Yeast 2 Hybrid Screen
  • 07:47Analysis of Clones from Selective Plates
  • 08:50Results: Yeast 2 Hybrid to Identify Binding Partners of Bacterial Effector Proteins
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

Bacterial effector proteins are important for establishing successful infections. This protocol describes the experimental identification of proteinaceous binding partners of a bacterial effector protein in its natural plant host. Identifying these effector interactions via yeast two-hybrid screens has become an important tool in unravelling molecular pathogenicity strategies.

Tags

Keywords: Bacterial EffectorNon-cultivable Plant PathogenYeast Two-hybrid ScreenCandidatus PhytoplasmaMalus DomesticaApple Proliferation DiseaseInfection ResearchDNA IsolationEffector Gene AmplificationBait VectorSelf-activationEffector ExpressionSD-trp PlateSD-trp MediumNMY51

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