Allogeneic Effector Cells
Allogeneic Effector Cells - Related Videos
06:48
Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
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Cited by 10 •
2014
Tail-skin transplantation is a powerful model for studying T cell-dependent rejection and tolerance induction during allogeneic immune responses in mice. The advantages of this protocol are minor invasive surgery, and ease of monitoring with no need to sacrifice the recipient mouse.
12:23
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
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Cited by 5 •
2015
Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.
08:52
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
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Cited by 49 •
2011
A rapid, simple and cost-effective protocol for the generation of donor-derived multivirus-specific CTLs (rCTL) for infusion to allogeneic hematopoietic stem cell transplant (HSCT) recipients at risk of developing CMV, Adv or EBV infections. This manufacturing process is GMP-compliant and should ensure the broader implementation of T-cell immunotherapy beyond specialized centers.
08:39
Electroporation of Functional Bacterial Effectors into Mammalian Cells
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Cited by 7 •
2015
Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.
04:41
Introduction of Bacterial Effector Proteins into Mammalian Cells by Electroporation
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2025
In this video, we demonstrate the introduction of bacterial effector proteins into mammalian cells by electroporation. The intracellular localization of the bacterial effector proteins is detected by confocal microscopy following staining with specific fluorophore-conjugated antibodies.
FAQs
Antigen recognition identifies cellular targets, while killer-cell receptor signaling translates that recognition into an immune response. Together, these processes can promote cytotoxicity, the directed damaging of target cells, or stimulate cytokine release. Their combined activity helps determine whether donor-derived cells respond to infected or abnormal cells in an experimental or recipient setting.
Host major histocompatibility complex molecules provide an important interaction point for donor-derived immune cells. Differences between donor and recipient systems can influence how those cells interpret host cells and whether activation occurs. This interaction is therefore central to studying immune compatibility, donor-recipient recognition, and the conditions that may lead to graft-versus-host reactions.
The outcome depends on how antigen recognition, receptor signaling, and host major histocompatibility complex interactions combine in the recipient or model system. Strong or misdirected activation may produce cytotoxicity, cytokine release, or graft-versus-host reactions, whereas controlled targeting can support responses against abnormal or infected cells. These contrasts make regulation important when evaluating therapeutic strategies.
Researchers place donor-derived immune cells in an experimental system containing infected or abnormal target cells and examine the resulting immune response. Measurements may focus on targeted cytotoxicity, cytokine release, or interactions with host compatibility molecules. This approach helps reveal how donor-recipient immune interactions influence recognition and response to cellular abnormalities or infection.
Studies using Allogeneic Effector Cells can evaluate how transferred donor-derived immune cells interact with a recipient and whether their activity remains appropriately targeted. The resulting observations inform research on adoptive cell therapy, immune compatibility, and the balance between effective responses and graft-versus-host reactions. They also provide a framework for examining how host molecules shape therapeutic immune activity.
They allow investigators to examine whether donor-derived immune activity can recognize and damage infected cells while limiting unwanted effects on host tissues. By tracking cytotoxicity, cytokine release, and donor-recipient interactions, researchers can assess both pathogen-directed responses and potential tissue injury. This makes the system useful for exploring immune control of infection alongside safety considerations.