Dendritic Cell Engineering
Dendritic Cell Engineering - Related Videos
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
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2023
This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult mouse.
21:08
Engineering Cell-permeable Protein
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Cited by 12 •
2009
Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.
06:39
Culture of myeloid dendritic cells from bone marrow precursors
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Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
FAQs
Antigen loading supplies dendritic cells with pathogen-derived material, whereas genetic or biochemical changes can adjust functions such as maturation, costimulatory signaling, cytokine production, or migration. These strategies therefore influence different stages of immune activation. Choosing between them depends on whether the study needs to provide antigen, regulate cellular behavior, or combine both goals to shape an antigen-specific response.
These features determine how effectively engineered dendritic cells support T-cell activation and influence the character of the resulting immune response. Maturation and costimulatory signaling affect the quality of communication with T cells, while cytokine production helps direct immune activity. Controlling them allows researchers to investigate or promote particular cellular and humoral responses rather than antigen presentation alone.
Migration positions engineered dendritic cells in lymphoid tissues, where they can interact with T cells after processing an antigen. This movement connects the initial antigen-handling step with immune activation at an appropriate site. Modifying migration can therefore help researchers examine how cell location influences antigen-specific responses and evaluate whether an engineered strategy supports effective immune priming.
After processing antigen, engineered dendritic cells present peptide-MHC complexes to T cells, initiating signals that can shape broader immune responses. Depending on the engineered antigen and regulatory features, the resulting activity may support cellular immunity, humoral immunity, or analysis of how the two are connected. This makes the cells useful for studying the direction and strength of antigen-specific protection.
A typical workflow begins by selecting a pathogen-derived antigen or a desired cellular property, then loading the cells or modifying them genetically or biochemically. Researchers next examine effects on antigen processing, peptide-MHC presentation, maturation, costimulatory signaling, cytokine production, or migration. The final interpretation links these engineered traits to T-cell activation and the intended immune outcome.
The approach is useful when vaccine research requires precise control over both antigen delivery and the signals that regulate immune activation. Researchers can test pathogen-derived antigens while adjusting maturation, costimulation, cytokine production, or migration. Comparing these engineered conditions helps identify strategies that promote antigen-specific protection and clarifies how cellular design influences vaccine-driven cellular or humoral immunity.
In infection research, engineered dendritic cells provide a controllable system for examining how pathogen-derived antigens are handled and presented to T cells. They can also help analyze host-pathogen interactions by separating antigen-related effects from changes in maturation, signaling, cytokine production, or migration. Beyond mechanism studies, the same approach informs cell-based therapies intended to improve antigen-specific protection.