Viable Cells
Viable Cells - Related Videos
05:26
Mechanical Dissociation: A Method to Obtain Viable Cells from a Tissue
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2023
This video describes the non-enzymatic dissociation of fresh human tissue to obtain viable cells. The technique is used for qualitative and quantitative analysis of CD45 positive cells (lymphocytes/leukocytes) present in various normal and malignant human tissues.
08:10
Capture and Release of Viable Circulating Tumor Cells from Blood
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Cited by 2 •
2016
A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.
07:32
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
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Cited by 36 •
2012
Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture purities.
02:30
Assessing Root Colonization of Tomato Seedlings by a Pathogenic Bacterium Using Viable Cell Counts
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2026
Source: Matthysse, A. G. Adherence of Bacteria to Plant Surfaces Measured in the Laboratory. J. Vis. Exp., (2018)This video demonstrates a protocol for measuring root colonization of tomato seedlings by wild-type and cellulose-deficient pathogenic bacteria. Viable counts of tightly adhering bacteria show greater adhesion and microcolony formation by the wild-type strain, highlighting cellulose’s role in stable root colonization.
12:38
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
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2008
This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.
FAQs
Membrane-integrity assays indicate whether cells preserve the boundary that allows them to exclude a vital dye. This readout is especially useful for identifying structural damage to cells after handling, infection, or treatment. It does not measure the same feature as enzyme-dependent activity, so membrane-based results should be interpreted according to the biological question being tested.
Metabolic assays evaluate whether active cellular enzymes convert an indicator substrate, providing a functional signal rather than a direct membrane readout. This approach can complement dye exclusion when researchers need evidence that cells remain biologically active. Comparing the two measurements helps clarify whether an experimental effect primarily involves membrane damage, reduced activity, or both.
Controlled conditions reduce the chance that culture variation or handling damage will be mistaken for an effect of the treatment being studied. Consistent viability measurements therefore help separate experimental cytotoxicity from losses caused during preparation or maintenance. This distinction improves interpretation of immune responses, infection-related effects, and other cell-based assay results.
In infection experiments, viable host or immune cells provide the functioning cellular context needed to examine pathogen entry, replication, cytotoxicity, inflammation, and antimicrobial responses. Monitoring cell condition alongside these outcomes helps researchers determine whether an observed change reflects infection-related biology or generalized cellular damage that could confound the assay.
A basic assessment selects a readout suited to the study, then measures either vital-dye exclusion or conversion of an indicator substrate by the cell sample. Performing the measurement under controlled conditions allows results from treated and comparison samples to be evaluated consistently. The resulting signal provides a basis for judging culture quality and experimental effects.
Viability measurements serve as a culture-quality check and help establish whether cells can carry out the biological functions under investigation. In immunology and infection research, this supports interpretation of inflammation, antimicrobial activity, pathogen-associated damage, and related responses. Detecting reduced viability also alerts researchers that handling or treatment may be influencing downstream assay outcomes.