Multi-gene Detection
Multi-gene Detection - Related Videos
22:10
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
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Cited by 3 •
2013
Structure-based drug design plays an important role in drug development. Pursuing multiple targets in parallel greatly increases the chance of success for lead discovery. The following article highlights how the Seattle Structural Genomics Center for Infectious Disease utilizes a multi-target approach for gene-to-structure determination of the PB2 influenza A subunit.
02:48
Loop-Mediated Isothermal Amplification for Bacterial Gene Detection
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2025
Source: Domesle, K. J., et al. Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation. J. Vis. Exp. (2020).This video demonstrates the loop-mediated isothermal amplification (LAMP) technique for detecting a target bacterial gene by amplifying DNA at a constant temperature. The reaction mixture is prepared by adding an isothermal master mix, specific primers for the target gene, and samples containing DNA templates. The...
10:01
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
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Cited by 2 •
2016
This work presents a simple and visual method to detect multi-nucleotide polymorphisms on a pneumatic droplet manipulation platform. With the proposed method, the entire experiment, including droplet manipulation and detection of multi-nucleotide polymorphisms, can be performed near 23 °C without the aid of advanced instruments.
06:21
Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
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2024
This protocol proposes holistic laboratory procedures required to detect single nucleotide polymorphisms in gastric cancer samples based on an ion semiconductor sequencing platform. The target sequences, ligating adapters, library amplification and purification, and quality control criteria are also described in detail.
10:17
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
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Cited by 9 •
2016
We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.
FAQs
Different assay designs capture multiple targets in parallel through primers, probes, or sequencing workflows. The resulting signals are then separated computationally so each gene, variant, or expression signal can be assigned within the combined profile. This coordinated capture-and-analysis process allows the assay to represent several molecular findings from one biological sample rather than treating each target as an isolated measurement.
The main analytical challenge is distinguishing signals that arise from different targets captured in the same assay. Computational analysis addresses this separation, allowing the final profile to preserve target-specific information while combining results. This matters because an undifferentiated signal would not show which gene, variant, or expression feature contributed to the observed molecular pattern.
A combined profile can reveal interacting alterations and heterogeneous disease signatures that may remain incomplete when targets are examined individually. This broader molecular view can improve diagnostic resolution by showing how several findings occur together. In medicine, the resulting pattern may support more individualized decisions than an interpretation based on one isolated gene or signal.
A workflow begins with a biological sample and selection of an assay capable of capturing several relevant genomic or expression targets. Primers, probes, or sequencing methods collect the target signals in parallel, after which computational analysis distinguishes and combines them. The output is a molecular profile that can be examined for diagnostic or treatment-related significance.
In hereditary disease screening, examining several genes can provide a broader assessment of potentially relevant genetic findings. In cancer profiling, the same strategy can capture multiple alterations and heterogeneous molecular signatures within the disease. These applications use the combined result to improve diagnostic resolution and help guide clinical interpretation beyond a single-target result.
For infectious-disease characterization, measuring several genetic targets can produce a broader molecular description of the biological sample. The combined pattern may distinguish more features of the disease state than a single-target measurement. This makes multi-gene detection relevant when clinical interpretation benefits from evaluating several genomic signals together rather than relying on one isolated marker.
Treatment-related interpretation depends on the combined molecular pattern rather than on one genetic signal alone. By revealing multiple alterations or expression features, the assay can provide information relevant to individualized clinical decisions. In cancer profiling and other medical contexts, this broader result may help connect molecular findings with treatment selection while preserving the distinct contribution of each detected target.