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Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

DOI:
10.3791/53830-v

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10:17 min

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January 31, 2016

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, , ,
1Department of Molecular Biosciences, The Wenner-Gren Institute (MBW),Stockholm University

Chapters

  • 00:05Title
  • 00:55Preparing the Embryos for Hybridization
  • 02:48Hybridization Probes to Prepared Embryos
  • 05:25Detecting the Probes and Staining
  • 07:59Results: Stain Precipitate Color Combinations
  • 09:42Conclusion

Summary

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We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.

Tags

Multi-targetChromogenicWhole-mount In Situ HybridizationGene ExpressionDrosophila EmbryosMRNA LocalizationMulti-colorGene Regulatory InteractionsEmbryonic DevelopmentOrganogenesis

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