Exogenous Insulin
Exogenous Insulin - Related Videos
05:52
Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
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Cited by 16 •
2011
Conserved insulin signaling pathways found in the fruit fly Drosophila melanogaster make this organism a potential tool for modeling metabolic disorders including type II diabetes. To this end, it is critical to establish physiological assays to effectively measure systemic insulin action in peripheral glucose disposal in the adult fly.
05:30
Exogenous Mitochondrial Transfer in Differentiating Brown Adipocytes and AGPAT2-Deficient Preadipocytes
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2026
Exogenous mitochondria of human and murine origin can be internalized by differentiating preadipocytes and tracked throughout adipogenic differentiation using imaging and genetic tools. While these mitochondria physically interact with the endogenous mitochondrial network, they do not increase the expression of markers associated with mature brown adipocytes.
05:35
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Cited by 4 •
2016
Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).
02:40
Inactivation of Bacteriophages Using Exogenous Lipopolysaccharide to Generate Phage-Free Salmonella Cultures
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2026
Source: Fernández-Fernández, R., et. al. Bacteriophage Removal from Infected Salmonella Cultures. J. Vis. Exp. (2024)This video demonstrates the use of exogenous LPS (Lipopolysaccharide) as a receptor mimic to inactivate lytic bacteriophages in Salmonella cultures. Through sequential incubation, centrifugation, and washing, phages are eliminated, resulting in a viable, phage-free bacterial suspension for downstream applications.
12:08
Improving IV Insulin Administration in a Community Hospital
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Cited by 7 •
2012
When compared to the previous paper protocol, implementation of a computerized glucose management system results in a substantial increase in blood glucose concentration measurements within the target range. Using a computerized glucose management system to monitor blood glucose levels, decreases in severe hypoglycemia (<40 mg/dL), clinical hypoglycemia (<70 mg/dL), and hyperglycemia (>180 mg/dL) also can be observed.
FAQs
After insulin binds its receptor, it can activate PI3K/Akt and MAPK signaling. These pathways provide a molecular link between external insulin administration and changes in cellular behavior. In neuroscience, examining this signaling helps researchers relate insulin availability to neuronal metabolism, synaptic activity, and cellular survival.
Insulin that reaches the brain can influence neural cells through insulin receptors. The resulting signaling connects metabolic information with neuronal processes, including energy use, synaptic function, and cellular maintenance. This makes receptor activity a useful focus for studying how changes in metabolic regulation may affect brain function.
Administered insulin provides a way to examine communication between metabolic systems and the nervous system. Researchers can investigate how an externally supplied hormonal signal affects neural signaling and behavior of brain cells. This approach supports broader study of neuroendocrine regulation, where metabolic status and neural activity are examined as connected processes.
Studies can assess how insulin-related signaling corresponds with neuronal metabolism, synaptic activity, and cellular survival. These outcomes represent different levels of neural function, from cellular energy handling to communication between neurons and maintenance of cell viability. Examining them together helps clarify how metabolic signals may influence overall brain function.
Researchers can use external insulin administration to examine how impaired insulin-related signaling may relate to cognition and brain function in the context of insulin resistance or diabetes. Comparing neural responses associated with insulin signaling helps connect metabolic dysfunction with neurological consequences and may identify processes relevant to altered cognitive or brain performance.
Findings can contribute to research on neuroendocrine regulation, synaptic plasticity, and therapies for metabolic or neurological disorders. The value of the approach lies in connecting insulin receptor signaling with neural outcomes rather than examining glucose regulation alone. This broader perspective may help explain how metabolic disturbances influence neural systems and guide therapeutic investigation.