Neural Tube Transplantation
Neural Tube Transplantation - Related Videos
12:48
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
0 Views •
Cited by 26 •
2012
Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.
07:37
Neural Tube Closure in Mouse Whole Embryo Culture
0 Views •
Cited by 22 •
2011
A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
09:03
Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
0 Views •
Cited by 10 •
2012
An approach for analyzing migration and eventual fate of avian neural crest cells in quail-chick chimeric embryos is described. This method is a simple and straightforward technique for tracing neural crest cells during migration and differentiation that are otherwise difficult to distinguish within an unmanipulated chick embryo.
02:17
Transplantation of Human Neural Progenitor Cells in the Mouse Brain
0 Views •
2025
Source: Weber, R. Z., et al. Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain. J. Vis. Exp. (2022)This video demonstrates the stereotaxic transplantation of genetically modified human neural progenitor cells (NPCs) into the mouse brain. Researchers inject NPCs expressing luciferase and fluorescent proteins, enabling in vivo bioluminescence imaging and post-mortem fluorescence microscopy to study cell survival, migration, and...
04:12
Stereotaxic Transplantation of Neural Cell Suspension into a Mouse with Traumatic Brain Injury
0 Views •
2025
Source: Furmanski, O., et al. Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells. J. Vis. Exp. (2019)In this video, human-induced pluripotent stem cell-derived neural cells are stereotaxically injected into the gray matter-white matter border of the deep cortex in a mouse model of traumatic brain injury. The transplanted cells integrate into the tissue, promoting functional recovery and circuit...
FAQs
The host environment can influence how transplanted neural tissue survives, differentiates, and integrates. Surrounding cells, signaling molecules, and developmental cues provide information that differs between locations, allowing researchers to examine how local conditions shape neural organization. Comparing grafts placed in different regions can therefore reveal the contribution of tissue surroundings to nervous system development.
Donor tissue carries a developmental history associated with its original region or embryo, while the host provides a new set of cues. Placing tissue in a different location allows researchers to examine how neural identity and body patterning reflect the interaction between intrinsic properties of the graft and signals from its new environment.
The experiment can examine several outcomes separately: whether the graft retains or changes its neural identity, whether it differentiates appropriately, and whether it integrates with surrounding tissue. These observations address different stages of development. A graft may provide evidence about local patterning cues, connectivity, or tissue organization rather than only its initial survival.
Connectivity and body patterning show whether transplanted tissue participates in organized nervous system development within its host location. Changes in these features can indicate how positional information and surrounding developmental signals influence tissue arrangement. Consequently, transplantation provides a way to study not only cell differentiation but also larger-scale organization of the developing nervous system.
A typical experiment selects embryonic neural tube tissue, identifies a defined location in the host, and surgically transfers the donor tissue there. Researchers then evaluate the graft in relation to its surroundings, focusing on survival, differentiation, integration, neural identity, connectivity, or patterning. The sequence links a precise tissue manipulation to developmental outcomes.
The donor's developmental region or embryo, the host location, and the relationship between the graft and surrounding tissue are central considerations. These choices determine which developmental cues the transplanted tissue encounters. Because local cells and signaling molecules may influence outcomes, defining the placement clearly is essential for interpreting differences in organization or differentiation.
This technique is useful when researchers need to test how nervous system development is organized by tissue interactions and developmental signals. It can help investigate neural identity, connectivity, and body patterning, while also providing experimental models relevant to regeneration and cell replacement strategies. Its value lies in linking tissue location with developmental and organizational outcomes.