Biophysical Stress

Biophysical stress is the physiological and behavioral strain caused by physical conditions such as temperature, pressure, radiation, mechanical forces, or limited energy. These conditions disturb homeostasis by altering cellular function, metabolism, sensory processing, and neuromuscular performance, prompting responses such as avoidance, reduced activity, changes in feeding, or altered social interactions. In behavioral research, measuring these responses helps reveal how organisms cope with environmental challenges and how stress influences survival, decision-making, and adaptation. The concept supports studies of animal welfare, ecology, evolution, and human health, while providing a framework for linking environmental conditions to observable behavior.

Biophysical Stress - Related Videos

Research

JoVE Journal - Behavior

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

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Cited by 3 •

2019

The purpose of this paper is to identify changes in stress levels after visitation to three different settings and to describe the methods used in identifying stress levels based on measures of salivary cortisol, α-amylase, and a psychological self-report...

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

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Cited by 7 •

2015

The mechanical properties and microstructure of the extracellular matrix strongly affect 3D migration of cells. An in vitro method to study the spatiotemporal cell migration behavior in biophysically variable environments, at both population and individual cell levels, is described.

Research

JoVE Journal - Biology
Free Sample

Isolation and Biophysical Study of Fruit Cuticles

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Cited by 16 •

2012

Aerial plant organs are protected by the cuticle, a supramolecular biopolyester-wax assembly. We present protocols to monitor selective removal of epi- and intracuticular waxes from tomato fruit cuticles on molecular and micro scales by solid-state NMR and atomic force microscopy, respectively, and to assess the cross-linking capacity of engineered cuticular biopolyesters.

Research

JoVE Journal - Biochemistry

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

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Cited by 2 •

2022

Cell-free reconstitution has been a key tool to understand the cytoskeleton assembly, and work in the last decade has established approaches to study septin dynamics in minimal systems. Presented here are three complementary methods to observe septin assembly in different membrane contexts: planar bilayers, spherical supports, and rod supports.

Stabilizing Protein and Protein—RNA Condensates in Low-Melting Agarose Hydrogels for Quantitative Biophysical Measurements

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2026

Here, we present a robust method to physically immobilize liquid–liquid phase-separated condensates within a porous low-melting agarose hydrogel matrix without disrupting their internal liquid dynamics. The approach minimizes droplet sedimentation, coalescence, and surface-wetting artifacts, enabling reproducible imaging and quantitative biophysical analysis over periods of hours.

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