Endo Painting
Endo Painting - Related Videos
07:46
Assessing Endothelial Vasodilator Function with the Endo-PAT 2000
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Cited by 165 •
2010
A noninvasive procedure to assess endothelial function is demonstrated using the Endo-PAT 2000.
02:58
Nanoparticle-Mediated Visualization of Endo-Lysosomal Remodeling in Bacterially-Infected Cells
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2025
Source: Zhang, Y., et al. Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria. J. Vis. Exp. (2015)This video demonstrates the use of fluorescent nanoparticles to visualize endo-lysosomal remodeling in human epithelial cells infected by intracellular bacteria. Following bacterial invasion, the pathogens manipulate the host cytoskeleton and endo-lysosomal system, forming vacuoles with tubular projections that alter vesicle transport.
09:57
2D and 3D Chromosome Painting in Malaria Mosquitoes
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Cited by 8 •
2014
Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).
09:54
Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
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Cited by 31 •
2011
Using specific glycosidases to remove sugars from glycoproteins followed by SDS-PAGE is a valuable method to detect glycan modifications on protein samples and is a good choice for initial glycobiology studies. Changes following deglycosylation can be detected as shifts in gel mobility or by staining with glycan sensitive reagents.
11:38
Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria
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Cited by 3 •
2015
This article describes methods for the synthesis and fluorescent labeling of nanoparticles (NPs). The NPs were applied in pulse-chase experiments to label the endo-lysosomal system of eukaryotic cells. Manipulation of the endo-lysosomal system by activities of the intracellular pathogen Salmonella enterica were followed by live cell imaging and quantified.
FAQs
The process depends on a sequence of coordinated events. Endothelial cells first attach to the prepared lumen surface, then spread to occupy available regions. Controlled fluid flow supports the development of a more continuous layer rather than leaving isolated cell patches. Together, these interactions determine how evenly the inner surface becomes covered and how closely the engineered conduit resembles a native vessel lining.
Continuous coverage creates a more complete biological interface between the blood-contacting material and the lumen. This supports the goal of making vascular scaffolds and conduits more similar to native vessels and can contribute to more biologically compatible cardiovascular devices. In bioengineering studies, the extent of coverage also provides a visible outcome for evaluating how effectively cells colonize the engineered surface.
The prepared condition of the lumen, the ability of endothelial cells to adhere, their capacity to spread, and the application of controlled fluid flow are central variables. These factors influence whether cells remain attached and develop into continuous coverage. Adjusting them helps researchers study cell-material interactions and identify conditions that improve endothelialization within a vascular scaffold or conduit.
A basic workflow begins with a prepared vascular scaffold or engineered conduit containing an accessible lumen. Endothelial cells are then introduced into that inner space so they can contact the surface, attach, and spread. Controlled fluid flow is applied to promote more continuous coverage. The resulting endothelialized lumen can then serve as a platform for vascular engineering or further investigation.
Researchers would apply this approach when developing tissue-engineered blood vessels, vascular grafts, or perfusable organ models that require an endothelialized inner surface. It is particularly relevant when a construct must interact with blood or support perfusion. The method helps move engineered conduits beyond purely material-based designs by incorporating a cellular layer that better reflects native vascular structure.
The approach provides a way to examine how endothelial cells respond to the inner surface of a vascular scaffold or conduit under controlled flow. Researchers can assess whether cells attach, spread, and form continuous coverage, linking material characteristics with biological outcomes. These observations can guide the design of more functional and biologically compatible cardiovascular devices and perfusable engineered tissues.