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JoVE Journal
Immunology and Infection

This content is Open Access.

2D and 3D Chromosome Painting in Malaria Mosquitoes
 

2D and 3D Chromosome Painting in Malaria Mosquitoes

Article doi: 10.3791/51173
January 6th, 2014 Usage Statistics

Summary January 6th, 2014

Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).

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