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Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria
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Chapters
- 00:05Title
- 01:26Synthesis of 10-nm Gold Nanoparticles (Gold NPs)
- 02:28Coating of Gold NPs with BSA and Labeling with Rhodamine N-hydroxysuccinimidyl Ester (NHS)
- 04:30Culture of HeLa Cells and Salmonella Bacteria
- 05:40Infection of HeLa Cells by Salmonella and Gold-BSA-Rhodamine NPs Pulse Chase-labeling
- 07:04Imaging and Image Analysis
- 09:18Results: Salmonella Infection Rearranges the Endolysosomal System in HeLa Cells
- 10:58Conclusion
This article describes methods for the synthesis and fluorescent labeling of nanoparticles (NPs). The NPs were applied in pulse-chase experiments to label the endo-lysosomal system of eukaryotic cells. Manipulation of the endo-lysosomal system by activities of the intracellular pathogen Salmonella enterica were followed by live cell imaging and quantified.
