Arabidopsis Genome

The Arabidopsis genome is the complete set of DNA in the model plant Arabidopsis thaliana, providing a reference for studying plant biology and genetics. Its genes and regulatory sequences are organized across five chromosomes, and genome sequencing, annotation, and targeted mutagenesis allow researchers to connect specific DNA regions with traits and biological processes. Because Arabidopsis has a small genome, rapid life cycle, and extensive genetic resources, scientists use it to investigate plant development, reproduction, metabolism, and responses to environmental stress. Findings from this system help clarify fundamental mechanisms and can inform research on agriculturally important crops.

Arabidopsis Genome - Related Videos

Research

JoVE Journal - Immunology and Infection

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

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Cited by 2 •

2013

Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.

A Method for Characterizing Embryogenesis in Arabidopsis

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Cited by 11 •

2017

This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.

Education

JoVE Core - Molecular Biology

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

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2020

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Lateral Root Inducible System in Arabidopsis and Maize

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Cited by 8 •

2016

The Lateral Root Inducible System (LRIS) allows for synchronous induction of lateral roots and is presented for Arabidopsis thaliana and maize.

Research

JoVE Journal - Biology
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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

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Cited by 143 •

2017

The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.

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