Fluorescence Signature
Fluorescence Signature - Related Videos
07:29
Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy
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Cited by 1 •
2020
Here, a protocol is presented for optically extracting and cataloging innate cellular fluorescence signatures (i.e., cellular autofluorescence) from every individual live cell distributed in a three-dimensional space. This method is suitable for studying the innate fluorescence signature of diverse biological systems at a single-cell resolution, including cells from bacteria, fungi, yeasts, plants, and animals.
09:11
Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures
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Cited by 1 •
2021
Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.
15:17
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Cited by 19 •
2011
We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.
12:09
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
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Cited by 12 •
2014
The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.
09:22
Introduction to Fluorescence Microscopy
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2023
Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently...
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The process begins when a fluorophore absorbs photons and moves into an excited electronic state. As it returns toward the ground state, it releases light with lower energy than the absorbed light. The distribution of emitted wavelengths creates a characteristic spectral pattern, while the amount of emitted light contributes information about the sample’s fluorescent response.
These conditions affect the fluorophore’s molecular environment and can change either the wavelengths emitted, the intensity of emission, or both. Consequently, the same fluorescent molecule may produce different patterns under different conditions. Such changes make fluorescence signatures useful for detecting environmental shifts, molecular interactions, and changes occurring within biological samples.
The emission spectrum can help indicate molecular identity because different fluorescent molecules or samples produce characteristic wavelength patterns. Emission intensity can provide information about abundance, although it also responds to surrounding conditions. Considering spectral pattern and intensity together helps researchers distinguish what is present from how much is present or how its environment has changed.
A basic workflow excites the fluorescent molecule or biological sample, observes the emitted light, and examines its wavelength distribution and intensity. Researchers then relate the resulting pattern to molecular identity, abundance, or environmental conditions such as pH, oxygen, or binding interactions. This workflow supports both spectral identification and monitoring of biological changes.
In microscopy, fluorescence signatures help researchers follow where fluorescent molecules or labeled biological features are located and how they change over time. The emitted pattern can therefore support measurements of localization and dynamics rather than simply showing that fluorescence is present. This makes the approach useful for examining spatial organization and changing cellular behavior.
Flow cytometry can use fluorescence signatures to characterize cells according to their emitted light patterns. Spectral differences help researchers identify or distinguish cellular populations, while changes in intensity can contribute information about the abundance of fluorescent molecules or markers. The method therefore connects optical measurements with biological classification and analysis of cellular state.
Biosensors can use changes in a fluorescence signature to report molecular interactions or shifts in local conditions. Because binding interactions and environmental factors can modify emission patterns or intensity, the signal can act as an indicator of a biological event. In this way, fluorescence-based systems help monitor molecular behavior and changes in cellular state.