Gal4-uas System
Gal4-uas System - Related Videos
06:29
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
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Cited by 11 •
2021
The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.
08:52
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
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Cited by 9 •
2015
Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.
13:34
Gene Trapping Using Gal4 in Zebrafish
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Cited by 19 •
2013
This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.
05:30
CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library
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Cited by 1 •
2024
We present a protocol for CRISPR-based modular assembly (CRISPRmass), a method for high-throughput construction of UAS-cDNA/ORF plasmid library in Drosophila using publicly available cDNA/ORF resources. CRISPRmass can be applied to editing various plasmid libraries.
12:51
Drosophila Development and Reproduction
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2023
One of the many reasons that make Drosophila an extremely valuable organism is that the molecular, cellular, and genetic foundations of development are highly conserved between flies and higher eukaryotes such as humans. Drosophila progress through several developmental stages in a process known as the life cycle and each stage provides a unique platform for developmental research. This video introduces each stage of the Drosophila life cycle and details the physical characteristics and major...
FAQs
Gal4 functions as the transcriptional activator, while UAS provides the regulatory DNA sequence recognized by Gal4. When Gal4 is present in a cell, it activates the responder gene linked to the UAS element. This division allows researchers to change the responder while retaining the same expression-control component, supporting flexible experiments on gene function and cell behavior.
The driver determines which cells contain Gal4 and, in inducible designs, when Gal4 activity occurs. Tissue-specific drivers restrict responder expression to selected cell populations, whereas inducible drivers provide temporal control. These distinctions help separate effects caused by a gene’s location of activity from effects caused by its timing, which is important when studying development, physiology, or behavior.
Its two-part arrangement separates expression control from the responder gene itself. A Gal4 driver supplies a defined spatial or temporal pattern, and a compatible UAS-linked responder supplies the experimental function, such as a reporter or altered gene activity. This modularity lets researchers apply different responders to the same expression pattern and compare distinct cellular effects.
Researchers first select a Gal4 driver with the desired tissue distribution or timing, then pair it with a UAS-linked responder suited to the question. The combined system is examined for expression or for changes produced by the responder. Results can reveal where and when the responder acts, and whether that activity changes cellular, physiological, behavioral, or disease-related outcomes.
A UAS-linked reporter can label cells that express the selected driver, helping investigators relate gene activity to particular tissues or neuronal populations. Other responders can alter gene activity or manipulate neuronal circuits. Comparing the resulting cellular, physiological, or behavioral changes helps connect specific cells and genetic functions with broader biological processes.
In Drosophila, researchers can combine defined Gal4 drivers with UAS responders to examine gene function in selected tissues or neuronal populations. The system supports studies of development, physiology, behavior, and disease while limiting responder activity in cells that lack Gal4. That restriction improves interpretation by linking observed effects to the targeted biological context rather than to the entire organism.