Programmable Freezers
Programmable Freezers - Related Videos
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
0 Views •
Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
14:00
Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
0 Views •
Cited by 5 •
2021
We describe a reliable method for the preparation of whole cell extracts from yeast or other cells using a cryogenic freezer mill that minimizes degradation and denaturation of proteins. The cell extracts are suitable for purification of functional protein complexes, proteomic analyses, co-immunoprecipitation studies and detection of labile protein modifications.
09:56
Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
0 Views •
2019
This protocol demonstrates how to use the Auto-CHO software for hierarchical and programmable one-pot synthesis of oligosaccharides. It also describes the general procedure for RRV determination experiments and one-pot glycosylation of SSEA-4.
10:28
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
0 Views •
Cited by 5 •
2017
This paper presents a series of protocols for developing engineered cells and functionalized surfaces that enable synthetically engineered E. coli to control and manipulate programmable material surfaces.
05:06
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
0 Views •
Cited by 10 •
2021
This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
FAQs
The programmed cooling rate controls how quickly a sample loses heat, helping reduce the formation of intracellular ice. Excessive intracellular ice can damage cells and tissues during freezing. By following a defined cooling schedule, the instrument creates more consistent conditions across samples, which supports improved viability and more reproducible recovery after preservation.
Cryoprotectants are used during controlled freezing to help limit damage associated with ice formation inside cells. Their role is especially important when preserving cell cultures, primary cells, embryos, or tissues. Combining cryoprotectants with a specified cooling rate gives researchers a standardized preservation strategy rather than relying on uncontrolled temperature changes.
Temperature records document whether a sample experienced the intended cooling program. This information supports reproducibility by allowing researchers to compare preservation conditions across experiments, samples, or storage batches. Recorded temperature changes also strengthen the consistency of biobanking and biomedical workflows, where reliable sample history can influence interpretation of later recovery and experimental results.
Researchers first establish a defined cooling program for the biological material and typically prepare the sample in the presence of cryoprotectants. The programmable freezer then applies and records the planned temperature changes. After controlled freezing, the preserved material can be transferred to long-term cryogenic storage, helping standardize the transition from preparation to extended preservation.
Programmable freezers support preservation of several biological materials, including cell cultures, primary cells, embryos, tissues, and other research samples. The approach is useful when maintaining viability and experimental consistency matters after freezing. Because different sample types may require controlled handling, the defined program provides a framework for applying reproducible cooling conditions across biological research workflows.
In biobanking, programmable freezers help standardize cooling conditions across stored samples, supporting consistent sample quality and recovery. In regenerative medicine and biomedical research, controlled preservation can help maintain the viability of cells and tissues needed for later studies. These capabilities contribute to more reliable experiments, organized sample storage, and improved confidence in downstream research outcomes.