Serratia Marcescens Detection
Serratia Marcescens Detection - Related Videos
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Differential Radial Capillary Action of Ligand Assay: A High-Throughput Technique to Identify Bacterial Nucleotide Second Messenger-Binding Intracellular Proteins
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Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside
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Cited by 8 •
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The cryosphere offers access to preserved organisms that persisted under past environmental conditions. A protocol is presented to collect and decontaminate permafrost cores of soils and ice. The absence of exogenous colonies and DNA suggest that microorganisms detected represent the material, rather than contamination from drilling or processing.
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Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
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This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
09:00
Detection of Protein Ubiquitination
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Cited by 96 •
2009
Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
FAQs
The red pigment prodigiosin can support recognition when it appears under suitable conditions, but its absence does not exclude the bacterium. Pigment production may be limited or ambiguous in a sample, so investigators combine colony assessment with biochemical or molecular confirmation. This layered approach reduces the chance of overlooking isolates that lack a visually characteristic appearance.
Prodigiosin provides a visible colony characteristic that can guide the initial assessment of cultured material. Its value is mainly presumptive rather than conclusive, because the overview identifies pigment production as condition-dependent and notes that pigment may be absent or ambiguous. Confirmation therefore requires additional biochemical or molecular evidence before identification is treated as reliable.
Culture reveals growth and colony features, whereas biochemical and molecular approaches examine additional characteristics. Biochemical testing contributes confirmation through bacterial properties, while species-specific molecular assays assess cellular or genetic markers. Using these methods after initial culture helps distinguish a tentative colony observation from a more supported identification, particularly when pigmentation does not provide a clear result.
Reliability depends on how well the available observations agree across the testing approaches. Colony appearance may be informative when prodigiosin develops, yet suitable conditions and pigment ambiguity can limit interpretation. Adding biochemical or species-specific molecular confirmation improves confidence by contributing independent evidence, making the final result more dependable for clinical, environmental, or laboratory samples.
A practical workflow begins with culturing the clinical, environmental, or laboratory sample and assessing the resulting colonies. Investigators then consider visible characteristics such as red pigment production, followed by biochemical or species-specific molecular confirmation when needed. Once identification is supported, antimicrobial testing or contamination tracking can provide the additional information required for the investigation.
The approach applies to environmental and laboratory materials as well as clinical samples. It can support monitoring of water, surfaces, and research materials, where identifying the organism may reveal contamination requiring further investigation. In clinical settings, the same identification process helps recognize opportunistic infections, while environmental use emphasizes persistence and potential transmission routes.
Reliable identification connects observable bacterial traits with broader biological questions, including transmission, persistence, and disease risk. In infection-control investigations, results can help track contamination and guide attention toward affected materials or settings. In research, combining colony traits with biochemical or genetic evidence provides a basis for relating detection outcomes to the organism’s behavior in clinical, environmental, and laboratory contexts.