Ultraviolet Disinfection
Ultraviolet Disinfection - Related Videos
09:21
Ultraviolet-Visible (UV-Vis) Spectroscopy
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Ultraviolet-visible (UV-Vis) spectroscopy is one of the most popular analytical techniques because it is very versatile and able to detect nearly every molecule. With UV-Vis spectroscopy, the UV-Vis light is passed through a sample and the transmittance of light by a sample is measured. From the transmittance (T), the absorbance can be calculated as A=-log (T). An absorbance spectrum is obtained that shows the absorbance of a...
01:30
Cleaning, Sterilization, and Disinfection
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2023
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease. Cleaning The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
01:02
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
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2024
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...
06:57
Determining Viral Disinfection Efficacy of Hot Water Laundering
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2022
In response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, a laboratory protocol was developed to test the viral disinfection efficacy of hot water laundering of cloth face coverings, cotton scrubs, and denim pants. The Phi6 virus (bacteriophage) was used as the organism to test disinfection efficacy.
03:20
Studying Chronic Exposure of Mice to Ultraviolet B Radiation
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Cited by 1 •
2025
The protocol describes a study of the systemic and local skin effects of Ultraviolet B exposure in mice.
FAQs
UV-C radiation is absorbed by microbial DNA or RNA, producing lesions such as pyrimidine dimers. These changes interfere with the genetic material’s ability to support replication, so affected microorganisms cannot reproduce effectively. The result is biological control through damage to replication processes rather than through adding chemical disinfectants.
Performance depends on the delivered dose, exposure time, and wavelength, along with conditions in the treated water, air, or on the surface. These variables determine how much germicidal radiation reaches microbial genetic material. Consequently, the same approach may produce different outcomes when the treatment environment or exposure conditions change.
Ultraviolet disinfection leaves no lasting residual on treated materials. After exposure ends, microorganisms introduced from the surrounding environment can contaminate the water, air, or surface again. This limitation matters in biological and public-health settings because treatment reduces microorganisms at the time of exposure but does not provide continuing protection afterward.
Ultraviolet disinfection controls microorganisms through physical exposure to germicidal radiation rather than by adding chemical disinfectants. Its lack of a lasting residual distinguishes it from approaches that leave disinfecting substances behind. That feature supports applications where chemical addition is not desired, while also requiring attention to possible recontamination after treatment.
Researchers first identify whether the target material is water, air, or a surface, then consider the dose, exposure time, wavelength, and relevant treatment conditions. These factors influence how effectively radiation reaches microbial DNA or RNA. Matching the treatment to the setting helps support control of microorganisms in laboratories and other biological environments.
Applications include laboratories, drinking-water treatment, healthcare environments, and food-processing areas. In these settings, the method supports control of bacteria, viruses, and other pathogens without adding chemical disinfectants. Its use is especially relevant when researchers or facility managers need to reduce microbial contamination in water, air, or on surfaces.