Viral Integrase
Viral Integrase - Related Videos
03:45
Purification of Viral Integrase Using Affinity Chromatography
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2026
Source: Lopez Jr., M. A., et al. Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase. J. Vis. Exp. (2017)This video demonstrates the purification of polyhistidine-tagged viral integrase using nickel affinity chromatography. The protocol uses imidazole gradient elution to selectively isolate the integrase based on its affinity for nickel-charged resin.
02:21
Purification of Viral Integrase Using Heparin Affinity Chromatography
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2026
Source: Lopez Jr., M. et al. Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase. J. Vis. Exp. (2017)This video demonstrates the procedure for purifying viral integrase from bacterial nuclease contamination using heparin Sepharose column chromatography followed by SDS-PAGE analysis to confirm protein purity.
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Cited by 21 •
2017
We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.
07:50
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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Cited by 10 •
2017
Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.
08:21
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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Cited by 11 •
2012
A quick and efficient method to integrate foreign DNA of interest into pre-made acceptor strains, termed landing pad strains, is described. The method allows site-specific integration of a DNA cassette into the engineered landing pad locus of a given strain, through conjugation and expression of the ΦC31 integrase.
FAQs
After viral DNA is newly synthesized, integrase recognizes its ends and performs 3′ processing, which removes short terminal nucleotides. It then catalyzes strand transfer, joining the processed viral DNA to host DNA. This ordered sequence prepares the viral ends for insertion rather than treating the DNA as an undifferentiated substrate.
Strand transfer places viral DNA into host DNA, but the integration process is not complete at that moment. Cellular repair pathways finish the remaining DNA-processing steps. Their participation explains why viral integration depends on cooperation between a viral enzyme and host-cell machinery, linking molecular virology to broader genome maintenance processes.
Integrase performs a required step in the insertion of viral genetic material into the host genome. Drugs that block integrase activity therefore focus on a specific molecular event rather than targeting the entire host cell. Studying this enzyme provides a foundation for developing antiretroviral treatments directed at viral genome integration.
Once viral genetic material becomes integrated into a host-cell genome, it can remain associated with that cellular genetic material. In some viruses, this persistence supports long-term replication. The connection between integration and persistence makes integrase especially important for understanding why certain viral infections can continue over extended periods.
Integrase studies reveal how viral DNA ends are recognized, processed, and inserted into host DNA. Researchers can use this molecular understanding when designing viral vectors for gene delivery. The same integration principles that explain viral persistence can therefore inform approaches intended to introduce genetic material into cells for research or therapeutic development.
Viral integrase provides a shared molecular context for studying infection, genome modification, and gene delivery. In infectious disease research, its activity helps explain viral persistence and supports antiretroviral drug development. In genome engineering and gene therapy research, understanding insertion into host DNA helps guide the design and study of viral delivery systems.