Electroporation Generator
Electroporation Generator - Related Videos
10:15
Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
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Cited by 5 •
2017
Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Cited by 7 •
2013
Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.
07:23
Electroporation of Craniofacial Mesenchyme
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2011
Craniofacial cartilages develop in close contact with other tissues and are difficult to manipulate in live animals. We are using electroporation to deliver molecular tools during growth of the craniofacial skeleton while bypassing early embryonic effects. This approach will allow us to efficiently test candidate molecules in vivo.
11:57
Electroporation of Mycobacteria
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Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
09:56
Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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2025
The efficiency of the Improved Genome Editing via Oviductal Nucleic Acids Delivery (I-GONAD) method is comparable to traditional microinjection, which requires zygote collection from donor females and transfer to pseudo-pregnant females. This protocol demonstrates its effectiveness by introducing CRISPR/Cas9-induced mutations into the ROSA26 locus on chromosome 6.
FAQs
These electrical variables determine how extensively cell membranes become permeabilized and how long the effect supports molecular entry. Increasing or changing pulse strength, duration, or number can alter delivery performance, but excessive conditions may reduce viability and recovery. Researchers therefore adjust the parameters together rather than treating any single setting as sufficient for every cell type.
Successful delivery requires enough membrane disruption for DNA, RNA, or other cargo to enter, while preserving sufficient cellular viability for recovery. If the electrical treatment is poorly matched to the cells, improved permeabilization may come at the expense of survival. This balance is especially important when cancer studies evaluate subsequent genetic or pharmacological responses.
Cell type is a major source of variation because cells can respond differently to the same electrical conditions. A pulse combination that supports useful delivery in one population may produce inadequate permeabilization or reduced viability in another. Consequently, researchers must optimize settings for the specific cells under investigation before comparing delivery or treatment-response results.
Researchers begin by selecting the cells and intended cargo, then vary pulse strength, duration, and number to identify conditions that provide membrane permeabilization while retaining enough viability for recovery. The selected settings should be evaluated for reproducibility in the same cell type. This optimization creates a more reliable basis for later gene-delivery or treatment-response experiments.
The transient membrane openings can support entry of DNA, RNA, and other therapeutic cargo. The appropriate cargo depends on the experimental objective, such as delivering genetic material for gene-delivery studies or introducing therapeutic material when investigating anticancer strategies. Because cell type and electrical conditions influence the result, cargo delivery should be assessed under optimized settings.
In cancer research, the instrument supports gene delivery, testing of anticancer strategies, and examination of how tumor cells respond to genetic or pharmacological treatments. Its value comes from combining controlled cargo entry with adjustable electrical conditions. Researchers can therefore investigate treatment effects in cells while accounting for the viability and recovery requirements of the experimental system.