Tumor Tissue Embedding
Tumor Tissue Embedding - Related Videos
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
03:39
Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC)
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2023
This protocol describes the preparation of formalin-fixed paraffin-embedded tumor samples which are used to preserve tissue and cytology samples from non-small cell lung cancer...
02:31
FFPE Tumor Tissue Macrodissection: A Technique to Obtain Specific Tumorous Tissue from Unstained Specimens
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2023
This video describes a method for the manual macrodissection of cancer tissue from unstained tissue specimens to obtain a specific cancer tissue. The tissue section can be processed further to obtain DNA for genome-wide studies on gastrointestinal cancers.
09:56
Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections
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2023
Source: Thomas Chaffee1, Thomas S. Griffith2,3,4, and Kathryn L. Schwertfeger1,3,4 1 Department of Lab Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455 2 Department of Urology, University of Minnesota, Minneapolis, MN 55455 3 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 4 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 Pathologic analyses of tissue sections can be used to obtain a better understanding of normal tissue...
08:49
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
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Cited by 6 •
2016
This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.
FAQs
Fixation, dehydration, clearing, and infiltration are sequential stages in paraffin-based preparation. Together, they convert a tumor specimen into a form that can be cooled into a firm block and sectioned. Keeping these stages consistent matters because the resulting sections are used to examine tumor architecture, cellular features, and biomarker expression across samples.
Frozen embedding uses a cryogenic medium and avoids paraffin processing, while paraffin embedding follows fixation, dehydration, clearing, and infiltration before the specimen is cooled into a block. The choice therefore changes the preparation route used for the tumor sample. Both approaches support sectioning for microscopic and molecular analysis, but they preserve and process tissue differently.
Orientation determines how the tumor is presented when sections are cut. Positioning the specimen consistently helps researchers examine tissue architecture and cellular features in comparable views rather than relying on inconsistent regions from different blocks. This supports more reproducible microscopic assessment and improves the reliability of comparisons among tumor specimens in cancer research.
A paraffin workflow begins with fixation, followed by dehydration, clearing, and infiltration with paraffin. The infiltrated specimen is then cooled to form a firm block that can be sectioned. Maintaining this sequence provides a consistent path from collected tumor tissue to sections suitable for histopathology, immunohistochemistry, and evaluation of molecular or biomarker-related features.
Sections from embedded tumor blocks support histopathology, immunohistochemistry, and assessment of tumor architecture, cellular features, and biomarker expression. These readouts help cancer researchers characterize disease and compare specimens. Embedded tissue can therefore support both microscopic examination and molecular analysis, allowing structural findings to be considered alongside biomarker-focused results.
Consistent embedding improves tissue quality and reproducibility, making comparisons between tumor specimens more dependable. In cancer studies, standardized preparations help researchers compare treatment responses and investigate mechanisms of tumor development. Reproducibility is especially important when differences in architecture, cellular features, or biomarker expression must be interpreted across samples processed for the same research purpose.