Etch Pit Density

Etch pit density is the number of chemically revealed etch pits per unit area on a crystal surface, providing a practical measure of crystallographic defect concentration and material quality. In this method, a specimen is exposed to a selective etchant that dissolves the lattice preferentially at dislocations or other defects, leaving microscopic pits that can be counted by optical or electron microscopy. Engineers use etch pit density to evaluate semiconductor wafers, bulk crystals, and epitaxial materials, compare growth or processing conditions, and identify defect levels that may affect electronic performance, mechanical reliability, and device yield.

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Research

JoVE Journal - Biology

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

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Cited by 6 •

2014

Basic techniques and refinements of freeze-fracture processing of biological specimens and nanomaterials for examination by transmission electron microscopy are described. This technique is a preferred method for revealing ultrastructural features and specializations of biological membranes and for obtaining ultrastructural level dimensional and spatial data in materials sciences and nanotechnology products.

Generating an Ultra-Low-Density Neuronal Culture Using a High-Density Neuronal Feeder Layer

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2025

This video demonstrates the method for culturing ultra-low-density neurons in the presence of a high-density neuronal feeder layer. It establishes a co-culture of varying-density neurons, ensuring close physical proximity. The growth factors secreted by high-density neurons help neuronal survival and growth, maintaining ultra-low-density neurons for a longer time period.

Electrochemical Etching and Characterization of Sharp Field Emission Points for Electron Impact Ionization

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2016

A method for electrochemically etching field emission tips is presented. Etching parameters are characterized and the operation of the tips in field emission mode is investigated.

Resorption Pit Assay: An In Vitro Technique to Quantify Calcium Resorption Activity of Mature Osteoclasts using Calcium Phosphate-Coated Culture Plates

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2025

In this video, we describe an in vitro osteoclastic resorption assay on calcium phosphate-coated cell culture plates cultured with human peripheral blood mononuclear cell-derived osteoclasts. The osteoclasts form a characteristic actin ring on the calcium phosphate-coated matrix of the cell culture plate and degrade the matrix within the actin ring region to release calcium and phosphate ions, resulting in the formation of distinct resorption pits within the degraded matrix.

Education

JoVE Science Education - Chemistry

Density Gradient Ultracentrifugation

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2023

Density gradient ultracentrifugation is a common technique used to isolate and purify biomolecules and cell structures. This technique exploits the fact that, in suspension, particles that are more dense than the solvent will sediment, while those that are less dense will float. A high-speed ultracentrifuge is used to accelerate this process in order to separate biomolecules within a density gradient, which can be established by layering liquids of decreasing density in a centrifuge tube. The...

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