Bioreporter Assay
Bioreporter Assay - Related Videos
02:36
The Luciferase Assay: A High-Throughput Assay to Measure Luminescent Signals Using Engineered Nanoluciferase System-Based Bioreporters
0 Views •
2025
The video demonstrates the luciferase assay used to quantify luciferase-tagged proteins. The assay uses an engineered luciferase enzyme composed of a large fragment that binds with the small fragment, which is conjugated with a protein of interest. The bound subunits form an active enzyme that, in the presence of the substrate, releases a luminescent signal. This signal is then quantified using a luminometer.
03:11
Bioreporter Assay: A Sensitive Technique using a Bioluminescent Reporter System to Detect SARS-CoV-2 Antibodies
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2025
In this video, we show the HiBit-RBD bioreporter system-based assay for detecting SARS-CoV-2 neutralizing antibodies. This system targets the viral RBD domain by using a bioluminescent reporter system containing HiBiT fused to the RBD domain.
13:16
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
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Cited by 5 •
2014
A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.
07:44
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
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Cited by 2 •
2021
The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.
08:12
The TUNEL Assay
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2023
One of the hallmarks of apoptosis is the nuclear DNA fragmentation by nucleases. These enzymes are activated by caspases, the family of proteins that execute the cell death program. TUNEL assay is a method that takes advantage of this feature to detect apoptotic cells. In this assay, an enzyme called terminal deoxynucleotidyl transferase catalyzes the addition of dUTP nucleotides to the free 3’ ends of fragmented DNA. By using dUTPs that are labeled with chemical tags that can produce...
FAQs
The stimulus first interacts with a responsive cellular, microbial, or enzymatic component. This activates a sensor pathway or regulatory element, which then controls a linked reporter gene. The resulting fluorescence, luminescence, color, or other detectable output provides a measurable indication that the relevant chemical or environmental condition has affected the biological system.
These measurements represent different aspects of environmental contamination. Presence indicates that a target pollutant or condition is detected, whereas bioavailability reflects whether it is accessible to the responsive biological system. Biological activity concerns whether the substance produces an effect in that system. A Bioreporter Assay can therefore add functional context beyond simply identifying chemicals.
A reporter response reflects how a biological system reacts to the combined conditions it encounters, rather than only listing individual chemical constituents. This makes the approach useful when mixtures contain several pollutants or biologically active compounds. The signal can help reveal whether the mixture produces a detectable biological effect, complementing information obtained through chemical analysis.
First, the responsive cells, microorganisms, or enzymes encounter the environmental sample or condition being examined. Next, the relevant sensor pathway or regulatory element may activate the reporter system. Finally, investigators measure the resulting signal, such as fluorescence, luminescence, or color, and interpret it as evidence of a corresponding biological response.
Researchers can use the assay when they need information about the biological significance of environmental contamination in addition to chemical identity. Chemical analysis can support identification of pollutants, while the biological response indicates whether detected substances are bioavailable or active in the test system. Together, the approaches support contamination assessment and environmental monitoring.
They can indicate whether samples contain metals, pesticides, toxic organic compounds, or other conditions that trigger a responsive biological system. Because the output is measurable, investigators can use it to assess contamination-related biological responses and examine effects from complex mixtures. This provides a functional perspective that strengthens interpretation of environmental chemical measurements.