Capillary Feeding
Capillary Feeding - Related Videos
02:13
Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster
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2023
This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).
08:50
Capillary Electrophoresis (CE)
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution. Analytes are separated due to differences in electrophoretic mobility, which varies with charge, solvent viscosity, and size. Traditional electrophoresis in gels is limited in the amount of voltage...
14:53
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Cited by 2 •
2017
Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.
03:45
Purifying Capillaries From a Porcine Brain
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2025
This video demonstrates the isolation of capillaries from fresh porcine brains. The process involves removing the meninges, isolating the grey matter from the brain tissue, homogenizing and filtering the grey matter to isolate the capillaries, and finally using enzymatic digestion to purify the capillaries further.
08:23
Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research
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Cited by 22 •
2013
Lyme disease is the most commonly-reported vector-borne disease in North America. The causative agent, Borrelia burgdorferi is a spirochete bacterium transmitted by Ixodid ticks. Transmission and detection of infection in animal models is optimized by the use of tick feeding, which we describe here.
FAQs
Volume change in the capillary serves as the primary quantitative signal. Researchers compare the starting and ending liquid levels after a defined feeding interval, converting the difference into an intake measurement. This approach turns feeding behavior into a standardized numerical readout, making it possible to examine whether infection, immune stimulation, or treatment changes consumption under controlled conditions.
Control of feeding time, solution composition, and environmental conditions is central to interpretation. A difference in measured consumption may reflect the experimental exposure, but it can also be shaped by changes in assay conditions. Keeping these factors consistent allows comparisons among subjects or treatment groups and helps link altered intake with host condition rather than uncontrolled variation.
Feeding intake provides a noninvasive behavioral and physiological readout that can change with host condition. Measuring consumption helps researchers examine how pathogens, immune stimuli, or treatments affect behavior without relying only on disease-associated observations. The resulting data can support analysis of relationships among altered feeding, infection susceptibility, pathogen exposure, and physiological changes linked to disease.
Because the assay produces a measured volume of consumed liquid, researchers can compare intake across experimental conditions while maintaining a defined feeding period. Reduced or increased consumption can then be evaluated alongside the exposure being tested, such as a pathogen, immune stimulus, or pharmacological treatment. This supports standardized assessment of whether an intervention is associated with altered feeding behavior.
A researcher presents the subject with liquid in a narrow, calibrated glass tube and permits feeding through the capillary opening. The feeding period, solution composition, and environmental conditions are controlled before intake is assessed from the change in liquid volume. Using the same setup across subjects or groups provides a consistent basis for comparing consumption.
The method is useful when investigators need to quantify feeding changes after pathogen exposure or when they want a standardized route for delivering infectious or pharmacological agents. Its intake measurement can connect behavioral changes with infection-related physiology. In this context, the assay supports experiments examining exposure, susceptibility, treatment effects, and disease-associated changes in the host.