Dendritic Cell Pulsing
Dendritic Cell Pulsing - Related Videos
05:16
A Vaccine-Pulsed Dendritic Cell-Based In Vitro Assay to Induce Lymphocyte Proliferation
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2025
This video demonstrates a monocyte-derived dendritic cell, MoDC-based assay to measure the immunogenicity of commercially available vaccines. MoDC assays investigate dendritic cell responses to antigens, pathogens, or treatments and can provide insights into immune responses, vaccine development, and immunotherapy research.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
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2023
This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult mouse.
12:43
Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
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Cited by 15 •
2014
Evaluated in syngeneic immunocompetent hosts, cancer stem cell (CSC) based dendritic cell (DC) vaccine demonstrated significantly higher antitumor immunity than traditional DC vaccines pulsed with heterogeneous bulk tumor cells.
FAQs
The antigen format determines how material becomes available for immune recognition. Peptides, proteins, lysates, and other antigen-containing preparations may differ in uptake, surface association, and processing before display on major histocompatibility complex molecules. Comparing these formats can reveal whether T-cell responses depend on the antigen itself, its processing requirements, or the conditions used for presentation.
Immature and mature dendritic cells can provide different experimental contexts for antigen loading and presentation. Immature cells may emphasize uptake, whereas mature cells can support studies of antigen display and downstream T-cell responses. Selecting the maturation state helps researchers examine how cellular status influences activation, cytokine production, or cytotoxicity in antigen-specific assays.
Antigen form, dose, and presentation conditions are key variables because each can alter how much target material reaches the display pathway and how effectively T cells recognize it. Researchers can vary these factors systematically, then measure antigen-specific activation, cytokine production, or cytotoxicity to determine which loading conditions produce the clearest or strongest response.
A typical workflow begins with immature or mature dendritic cells and an antigen-containing material such as a peptide, protein, or lysate. The cells are incubated with the selected material to permit uptake or surface association, after which antigen processing and display can be assessed through interaction with T cells and measurement of immune responses.
The principal outcomes are antigen-specific T-cell activation, cytokine production, and cytotoxicity. Together, these readouts show whether pulsed dendritic cells can stimulate recognition and functional responses rather than merely associate with antigen. Comparing results across antigen preparations or loading conditions helps characterize the quality and magnitude of the adaptive immune response.
In infection research, pulsed dendritic cells support investigations of immune recognition of pathogen-derived targets and the responses generated against them. The approach also contributes to vaccine studies by testing how antigen form, dose, and presentation conditions shape adaptive immunity. In some experiments, the cells serve as an experimental cellular vaccine platform for evaluating candidate immune responses.