Ex Vivo Wound Model
Ex Vivo Wound Model - Related Videos
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
09:15
Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model
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Cited by 8 •
2017
Here we describe a novel diabetic murine model utilizing hairless mice for real-time, non-invasive, monitoring of biofilm wound infections of bioluminescent Pseudomonas aeruginosa. This method can be adapted to evaluate infection of other bacterial species and genetically modified microorganisms, including multi-species biofilms, and test the efficacy of antibiofilm strategies.
02:13
Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen
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2025
The video demonstrates the generation of an ex vivo ovine skin model infected with Staphylococcus aureus. This entails treating a lamb's forehead skin biopsy section with antibiotics, establishing a wound bed, positioning it on a permeable membrane in a multiwell plate with antibiotic-free medium, and establishing bacterial infection in the skin wound.
05:39
Murine Model of Wound Healing
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Cited by 207 •
2013
A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.
11:12
Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
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Cited by 10 •
2013
The embryonic epidermis of very late stage Drosophila embryos provides an in vivo system for rapid puncture wound response analysis and can be combined with genetic manipulations or chemical microinjection treatments to advance studies in wound healing for translation into mammalian models.
FAQs
Biological relevance comes from maintaining several tissue-level contributors together: resident cells, extracellular matrix, immune signaling, and microbial interactions. Rather than isolating one pathway, the model allows these features to be monitored as wound conditions change over time. This makes it possible to relate host defense signals to pathogen behavior and repair-related responses within the same experimental system.
The model occupies an intermediate position between simplified cell cultures and complex in vivo studies. It retains tissue organization and resident biological components that isolated cells may not reproduce, while offering more controlled experimental conditions than a whole organism. This balance supports focused investigation of infection and repair processes while reducing reliance on whole-animal experiments.
Resident cells and extracellular matrix provide tissue context for interpreting immune and microbial behavior. Their presence allows researchers to monitor how immune signaling and pathogen interactions occur within viable wounded tissue rather than in an isolated cell population. Consequently, observed inflammatory or repair-related responses can be evaluated alongside changes in microbial activity and tissue condition.
Establishing the system requires excised viable tissue, creation of a standardized wound, and maintenance under controlled culture conditions. Researchers then monitor the wounded tissue over time, examining immune signaling, microbial interactions, pathogen growth, or repair-related changes. Standardizing the wound and culture environment helps make responses more comparable across experimental conditions.
Researchers can evaluate inflammatory responses, pathogen growth, antimicrobial treatment effects, and tissue-repair processes. Because these features can be monitored over time in the same cultured tissue system, the model supports comparisons between infected and treated conditions. The resulting observations help characterize host defense activity and assess how interventions influence microbial and wound-related outcomes.
This approach is useful when investigators need a controlled platform for studying infection mechanisms or testing therapeutic strategies while preserving relevant tissue biology. It can connect immune signaling with microbial interactions and wound repair, providing context that simplified cultures may lack. Its use also supports efforts to reduce reliance on whole-animal experiments before more complex in vivo studies.