/
/
Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:13Diabetes Induction and Surgical Preparation
- 03:12Wound Surgery
- 04:36Biofilm Inoculum Infection, Bioluminescence Expression Assessment, and Wound Measurement and Imaging
- 07:04Results: Representative Bioluminescence Expression, Biofilm Evolution, and Wound Closure Analyses
- 08:08Conclusion
Here we describe a novel diabetic murine model utilizing hairless mice for real-time, non-invasive, monitoring of biofilm wound infections of bioluminescent Pseudomonas aeruginosa. This method can be adapted to evaluate infection of other bacterial species and genetically modified microorganisms, including multi-species biofilms, and test the efficacy of antibiofilm strategies.
