Peg Plate Method
Peg Plate Method - Related Videos
06:41
A Peg Plate Biofilm Assay for Screening Antibacterial Agents
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2025
This video demonstrates an assay for screening antimicrobial compounds that efficiently eliminate biofilms using a peg-plate. The methodology monitors the biofilm's metabolic state after treatment with antimicrobial compounds, facilitating the determination of the minimum biofilm eradication concentration.
07:50
Electrophysiology of Scorpion Peg Sensilla
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Cited by 4 •
2011
This article describes an electrophysiological method for isolating chemical stimulation to individual sensilla via extracellular, tip-recordings under mineral oil.
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
04:54
Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
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Cited by 60 •
2011
A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation1.
06:56
Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
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Cited by 17 •
2013
Poly(ethylene glycol) (PEG) brush-arm star polymers (BASPs) with narrow mass distributions and tunable nanoscopic sizes are synthesized in via ring opening metathesis polymerization (ROMP) of a PEG-norbornene macromonomer followed by transfer of portions of the resulting living brush initiator to vials containing varied amounts of a rigid, photo-cleavable bis-norbornene crosslinker.
FAQs
Microorganisms first attach to the peg surfaces while those surfaces remain immersed in culture wells. As incubation continues, the attached cells produce extracellular polymeric substances, or EPS, which contribute to the structured organization of the community. This arrangement creates a consistent surface-associated system for comparing biofilm growth across different microorganisms or experimental conditions.
Washing removes residual material before the peg-bearing lid is transferred to fresh media, helping focus subsequent analysis on microorganisms associated with the peg surfaces. Fresh media then provides a separate condition for evaluating the established biofilm. This sequence supports measurements of growth, viability, or response to antimicrobial treatment without returning the lid to the original culture wells.
The method allows standardized comparisons among clinical isolates, host-associated pathogens, and infection-relevant conditions. Researchers can examine how these biological sources or conditions influence biofilm persistence, growth, and treatment response. Because multiple pegs are handled through a shared microtiter plate format, the approach is suited to high-throughput comparisons rather than isolated single-sample observations.
A typical workflow places microbial cultures in the wells of a microtiter plate and fits the specialized peg lid over them. During incubation, microorganisms attach to the immersed pegs and develop surface-associated communities. The lid is then washed and transferred to fresh media, where researchers assess biofilm growth, viability, or susceptibility to an antimicrobial treatment.
After the peg-associated communities have developed, the method can support assessment of biofilm growth and viability, as well as susceptibility to antimicrobial treatment. These readouts help distinguish whether an experimental condition is associated with persistence or reduced survival. The resulting comparisons can clarify how treatment responses vary among microbial samples or infection-relevant conditions.
In immunology and infection studies, the technique provides a standardized platform for examining host-associated pathogens and clinical isolates under infection-relevant conditions. Researchers can use the resulting comparisons to investigate persistence and treatment response, two features linked to biofilm-associated disease. Its high-throughput format also makes it practical for evaluating multiple samples or conditions within the same experimental framework.