Fluorescent Nanoparticles
Fluorescent Nanoparticles - Related Videos
07:31
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
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Cited by 13 •
2014
Analysis of nanoparticle interaction with defined subpopulations of immune cells by flow cytometry.
07:41
Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
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Cited by 1 •
2016
Here we present a reliable method to monitor the incorporation of nanoparticles into a polymer host matrix via swell encapsulation. We show that the surface concentration of cadmium selenide quantum dots can be accurately visualized through cross-sectional fluorescence imaging.
08:14
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
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Cited by 1 •
2015
This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.
08:17
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
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Cited by 24 •
2011
Fluorescent nanoparticles produced in our lab are used for imaging ion concentrations and ion fluxes in biological systems such as cells during signaling and interstitial fluid during physiological homeostasis.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
FAQs
The emitting component helps determine the optical behavior of these particles. Semiconductor quantum dots, fluorescent dyes, and doped inorganic materials can each provide the basis for fluorescence. Because their signals are tunable and bright, selecting among these components can support imaging strategies that require visual contrast or differentiation.
Surface functionalization serves two related purposes in biological settings. It can improve water dispersibility, helping particles remain distributed in aqueous environments, and it can add features that target specific cells or biomolecules. These changes connect particle design with biological specificity when signals must be associated with a selected cellular or molecular target.
Size, optical stability, and the ability to modify the surface are central design variables. These properties can influence how effectively particles support biological measurements and can enhance sensitivity. They also enable multiplexed measurements, in which multiple signals or targets are assessed within the same broader experimental approach.
Their value extends beyond producing a visible signal. Fluorescent nanoparticles can support high-contrast imaging of biological structures and processes, help detect biomarkers, track cells, and monitor drug delivery. These applications allow researchers to connect fluorescence with specific biological events, making the particles useful across imaging and molecular investigation.
In cell tracking, the particles provide fluorescent signals that help researchers follow labeled cells during an investigation. For drug-delivery studies, they can support monitoring of where delivery-related processes occur. Their tunable signals and modifiable surfaces make it possible to relate detected fluorescence to selected cells, biomolecules, or delivery behavior.
Clinical translation requires attention to toxicity, tissue penetration, and clearance. A particle may produce a strong signal yet remain unsuitable if it creates harmful effects, cannot penetrate the relevant tissue, or is not adequately cleared. These considerations determine whether the imaging or detection advantages can be applied safely in medical settings.