Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Harald Lahm; Stefanie A. Doppler; Martina Dre&#223;en; Klaudia Adamczyk; Marcus-Andr&#233; Deutsch; Hanna Ulrich; Matthias Schiemann; R&#252;diger Lange; Markus Krane

doi:10.3791/53268

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Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

11,010 Views

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08:14 min

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November 13, 2015

DOI:

10.3791/53268-v

DOI:

10.3791/53268-v

•

08:14 min

November 13, 2015

•

7 Views

Harald Lahm*¹, Stefanie A. Doppler*¹, Martina Dreßen¹, Klaudia Adamczyk¹, Marcus-André Deutsch¹, Hanna Ulrich², Matthias Schiemann^2,3, Rüdiger Lange^1,4, Markus Krane^1,4

¹Department of Cardiovascular Surgery, German Heart Center Munich,Technische Universität München, ²Institute for Medical Microbiology, Immunology, and Hygiene,Technische Universität München, ³Clinical Cooperation Groups: “Antigen-specific Immunotherapy” and “Immune Monitoring”, Helmholtz Center Munich (Neuhererg),Technische Universität München, ⁴DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance

Summary

This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.