This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.
Lahm, H., Doppler, S. A., Dreßen, M., Adamczyk, K., Deutsch, M., Ulrich, H., Schiemann, M., Lange, R., Krane, M. Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles. J. Vis. Exp. (105), e53268, doi:10.3791/53268 (2015).