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Immunology and Infection
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Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

DOI:
10.3791/51345-v

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07:31 min

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March 28, 2014

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, , , , , ,
1Center for Micro-BioRobotics @SSSA,Istituto Italiano di Tecnologia, 2Department of Biology,University of Pisa, 3Center for Biomolecular Nanotechnologies @UniLe,Istituto Italiano di Tecnologia

Chapters

  • 00:05Title
  • 00:56Nanoparticle Internalization in Blood Leukocytes and Purified Monocytes
  • 02:22Flow Cytometric Analysis
  • 03:44Results: Representative FITC-SiO2 Nanoparticle Internalization Analyses
  • 06:55Conclusion

Summary

Automatic Translation

Automatic Translation

Analysis of nanoparticle interaction with defined subpopulations of immune cells by flow cytometry.

Tags

Keywords: Flow CytometryNanoparticle InteractionImmune CellsFITC-SiO2 NanoparticlesRhodamine-SiO2 NanoparticlesPrimary MicrogliaGFP-expressing Monocyte/macrophage LineageCell FluorescenceSide-scattered Light

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