Head Upright Tilt
Head Upright Tilt - Related Videos
07:34
Upright Imaging of Drosophila Embryos
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Cited by 7 •
2010
Here we present a mounting protocol for stained Drosophila embryos in an upright position that allows imaging of cross-sections using Confocal microscopy.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
14:09
Tilt Testing with Combined Lower Body Negative Pressure: a "Gold Standard" for Measuring Orthostatic Tolerance
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Cited by 31 •
2013
We describe a "gold standard" for evaluating orthostatic tolerance (OT) using tilt testing with combined lower body negative pressure (LBNP). This can be combined with non-invasive evaluations of cardiovascular reflex control. Normal and abnormal responses are defined.
02:32
Generation of Tilted Amygdala Slices from a Mouse Brain
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2025
The video demonstrates the generation of tilted amygdala slices from a mouse brain. The brain is positioned at an angle on the vibratome specimen stage to obtain slices containing the amygdala and axons from the medial prefrontal cortex that form synaptic connections with the amygdala. The brain slices are maintained at the physiological temperature to preserve the tissue for further experiments.
09:47
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
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Cited by 13 •
2015
The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.
FAQs
The key challenge is maintaining circulation when gravity shifts blood toward the legs. Venous return and cardiac filling can decrease, leaving less blood available for the heart to pump. The resulting cardiovascular response must preserve blood pressure and cerebral perfusion, which explains why changing posture can expose abnormalities that may not appear while a person remains lying down.
Autonomic reflexes coordinate adjustments in heart rate and vascular tone when cardiac filling falls. These changes help counter the effects of blood displacement caused by upright posture and support blood pressure and blood flow to the brain. An inadequate response can provide evidence of impaired autonomic regulation in a patient with compatible symptoms.
If cardiovascular adjustments do not sufficiently preserve blood pressure or cerebral perfusion, the person may experience symptoms after moving toward an upright position. The clinically important finding is the relationship between the posture change, recorded cardiovascular changes, and reported symptoms. This relationship can help distinguish a meaningful orthostatic response from an isolated measurement.
Clinicians record blood pressure and pulse while the person progresses from lying toward an upright posture, while also noting symptoms. Examining these variables together shows whether cardiovascular responses accompany the positional change and whether the patient reports a relevant clinical reaction. The combined record supports interpretation rather than relying on symptoms or one measurement alone.
The assessment can contribute to the evaluation of unexplained syncope, orthostatic hypotension, and related autonomic dysfunction. It is most useful when clinicians need to examine how a positional challenge affects cardiovascular regulation and symptoms. Findings do not replace broader clinical reasoning, but they can help identify a response pattern requiring further evaluation.
Changes in blood pressure, pulse, and symptoms provide evidence about how the cardiovascular system responds to an upright challenge. Clinicians can use this pattern to support diagnostic reasoning in patients with fainting or orthostatic complaints. The results may also guide decisions about further evaluation or management, particularly when they align with the patient’s clinical history.