Ige-mediated Allergy
Ige-mediated Allergy - Related Videos
10:22
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
0 Views •
Cited by 14 •
2011
Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.
09:40
Detection of True IgE-expressing Mouse B Lineage Cells
0 Views •
Cited by 3 •
2014
In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.
07:22
Basophil Activation Test for Allergy Diagnosis
0 Views •
Cited by 9 •
2021
The basophil activation test is a complementary in vitro diagnostic test for the evaluation of IgE-mediated allergic reactions based on the detection of basophil activation in the presence of a specific stimulus through the measure of activation markers by flow cytometry.
04:34
Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation
0 Views •
Cited by 44 •
2018
Here we present a new method to accurately measure body temperature differences in passive systemic anaphylaxis (PSA) and food allergy mouse models using an infrared thermometer. This procedure has been accurately duplicated in previous PSA results.
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
0 Views •
Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
FAQs
Prior sensitization establishes allergen-specific IgE on mast cells and basophils through high-affinity FcεRI receptors. A later encounter can therefore engage antibody already positioned on these immune cells, rather than requiring the response to begin from scratch. This explains why subsequent exposure can produce an immediate reaction.
FcεRI is the high-affinity receptor that anchors allergen-specific IgE to mast cells and basophils. When an allergen cross-links the receptor-bound antibodies, these cells degranulate rapidly. That cellular event connects recognition of the allergen with release of histamine, leukotrienes, and other inflammatory mediators responsible for the early response.
Mast-cell and basophil mediators, including histamine and leukotrienes, convert antibody recognition into inflammation and symptoms. The same underlying pathway can contribute to allergic rhinitis, asthma, urticaria, or anaphylaxis, depending on the clinical presentation. Their rapid release helps explain the immediate nature and potentially severe effects of the response.
These categories represent different possible triggers for the same immediate hypersensitivity pathway. Considering foods, drugs, and environmental allergens helps connect a patient’s suspected exposure with allergen-specific IgE testing and the relevant clinical condition. This broad trigger assessment is important because the source of exposure can guide subsequent allergen avoidance.
Allergen-specific IgE testing detects antibodies directed against particular allergens, providing diagnostic information about the immune response. Because the suspected trigger may be a food, drug, or environmental substance, testing can help evaluate which allergen is associated with the presentation. Its value is greatest when interpreted within the broader clinical assessment.
Management can target different points in the clinical problem. Allergen avoidance reduces contact with the suspected trigger, antihistamines address effects associated with histamine, epinephrine is included when anaphylaxis is a concern, and targeted biologic therapies provide another treatment strategy. The appropriate approach depends on the condition and potential severity.
The pathway is relevant to allergic rhinitis, asthma, urticaria, and anaphylaxis. These conditions demonstrate the range of outcomes associated with immediate hypersensitivity, from allergic disease affecting particular clinical presentations to potentially severe systemic reactions. Recognizing this shared immune context helps connect diagnostic testing with treatment planning in medicine.